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Test Name:
F-Actin IgA Antibody

• SBMF No:
  45572
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  ACTIN IGA
• ABN:
  Not required
• CPT Code:
  83516
• LOINC Code:
  19146-0
• Ref Lab Test No:
  0051724
• Also Known As:
  Smooth Muscle IgA Antibody, by ELISA
• Also See:
  35198 Celiac Serology Profile
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  0.5 mL
• Min Vol:
  0.3 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  After separation from cells:
  48 hours room temperature (20-30°C)
  2 weeks refrigerated (2-8°C)
  1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Plasma sample
  Icteric, contaminated, severely hemolyzed, or lipemic sample
• Methodology:
  Enzyme-Linked Immunosorbent Assay (ELISA)
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  20.0 Units or less: Negative
  20.1 - 24.9 Units: Equivocal
  25.0 Units or greater: Positive

  Interpretive Data:
  The presence of IgA antibodies against F-Actin in patients having positive serologic markers for celiac disease, such as anti-endomysial, anti-transglutaminase, and/or anti-gliadin peptide IgA, indicates the presence of intestinal villus atrophy. Positive results should be confirmed with biopsy.

• Day Run:
  Tue
• Time Reported:
  2-9 days
• Test Type:
  IMMUNOLOGY