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Test Name:
Bordetella pertussis IgA and IgG Antibodies, by ELISA with Immunoblot if Indicated

• SBMF No:
  45564
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  BORD PAN2
• ABN:
  Required – Not FDA-Approved
• CPT Code:
  86615x2
  If indicated, add 86615 for each immunoblot
• LOINC Code:
  42328-5; 42330-1
• Ref Lab Test No:
  2001774
• Test Includes:
  B. pertussis IgA Antibody, ELISA
  B. pertussis IgA Antibody, Immunoblot (If Indicated)
  B. pertussis IgG Antibody, ELISA
  B. pertussis IgG Antibody, Immunoblot (If Indicated)
• Also Known As:
  Pertussis IgA and IgG Antibodies
• Also See:
  44271 Bordetella pertussis IgA, IgG, and IgM Antibodies, by ELISA with Immunoblot if Indicated
  44752 Bordetella pertussis IgG Antibody, by ELISA with Immunoblot if Indicated
  44758 Bordetella pertussis IgM Antibody, by ELISA with Immunoblot if Indicated
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  1.0 mL
• Min Vol:
  0.3 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
  Parallel testing is preferred
  Label samples as: "acute" or "convalescent"
  Convalescent samples must be received within 30 days from receipt of acute samples
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  After separation from cells:
  48 hours room temperature (20-30°C)
  2 weeks refrigerated (2-8°C)
  1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Contaminated, heat-inactivated, or severely lipemic sample
• Spec Remarks:
  Separate serum from cells ASAP
• Methodology:
  Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
  Qualitative Immunoblot
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  Bordetella pertussis Antibody, IgA by ELISA
  0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgA antibody.
  1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
  1.2 U/mL or greater: Positive - IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.

  Bordetella pertussis Antibody, IgA by Immunoblot
  Negative

  Bordetella pertussis Antibody, IgG by ELISA
  0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgG antibody.
  1.0-2.4 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
  2.5 U/mL or greater: Positive - IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.

  Bordetella pertussis Antibody, IgG by Immunoblot
  Negative

  Note:
  If Bordetella pertussis Antibody, IgA by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added.

• Additional Test Info:
  This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
• Day Run:
  Tue
• Time Reported:
  2-9 days
• Test Type:
  INFECTIOUS ANTIBODY