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Test Name:
Diphtheria, Tetanus, and H. Influenzae b IgG Antibodies


  • SBMF No:
    45553
  • Performance Lab Name:
    ARUP Laboratories
  • Test Mnemonic:
    DTH
  • ABN:
    Not required
  • CPT Code:
    86317x3
  • LOINC Code:
    13227-4; 6367-7; 11256-5
  • Ref Lab Test No:
    0050779
  • Test Includes:
    Diphtheria IgG Antibody
    Haemophilus influenzae b IgG Antibody
    Tetanus IgG Antibody
  • Also Known As:
    Immune Response
    Vaccine Response
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Pref Vol:
    1.5 mL
  • Min Vol:
    0.45 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum to separate plastic tube
    Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine"
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    After separation from cells:
    2 days room temperture
    2 weeks refrigerated (2-8°C)
    1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Plasma or body fluid sample
    Contaminated, hemolyzed or, severely lipemic sample
  • Spec Remarks:
    Separate serum from cells ASAP or within 2 hours of collection
    "Pre" and "post" vaccination specimens should be submitted together for testing.
    "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen.
  • Methodology:
    Quantitative Multi-Analyte Fluorescence Detection (MAFD)
  • Clinical Significance:
    Refer to:
    ARUP Consult | The Physician's Guide to Laboratory Test Selection and Interpretation
  • Reference Range:

    Diphtheria and Tetanus
    Antibody concentration of > 0.1 IU/mL is usually considered protective for diphtheria or tetanus

    Haemophilus influenzae type B
    < 1.0 µg/mL = Antibody concentration not protective
    ≥ 1.0 µg/mL = Antibody to H. influenzae type B detected. Suggestive of protection.

    Interpretive Data:
    Responder status is determined according to the ratio of a one-month post-vaccination sample to pre-vaccination concentration of IgG antibodies as follows:

    Diphtheria and tetanus:
    1. If the post-vaccination concentration is less than 1.0 IU/mL, the patient is considered a nonresponder.
    2. If the post-vaccination concentration is greater than or equal to 1.0 IU/mL, a patient with a ratio of less than 1.5 is a nonresponder, a ratio of 1.5 to less than 3.0, is a weak responder, and a ratio of 3.0 or greater, is a good responder.
    3. If the pre-vaccination concentration is greater than 1.0 IU/mL, it may be difficult to assess the response based on a ratio alone. A post-vaccination concentration above 2.5 IU in this case is usually adequate.

    Haemophilus influenzae B:
    1. If the post-vaccination concentration is < 3.0 µg/mL, the patient is considered to be a nonresponder.
    2. If the post-vaccination concentration is 3.0 µg/mL, a patient with a ratio of 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder

  • Day Run:
    Sun-Sat (daily)
  • Time Reported:
    2-3 days
  • Test Type:
    INFECTIOUS ANTIBODY

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