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Test Name:
F-Actin IgG Antibody

• SBMF No:
  45528
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  ACTIN IGG
• ABN:
  Not required
• CPT Code:
  83516
• LOINC Code:
  44706-0
• Ref Lab Test No:
  0055248
• Also Known As:
  Smooth Muscle IgG Antibody, by ELISA
• Also See:
  28151 Smooth Muscle Antibody
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  0.5 mL
• Min Vol:
  0.3 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  After separation from cells:
  48 hours room temperature (20-30°C)
  2 weeks refrigerated (2-8°C)
  1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic sample
  Urine or plasma samples
• Spec Remarks:
  Separate serum from cells ASAP
• Methodology:
  Enzyme-Linked Immunosorbent Assay (ELISA)
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  19 Units or less: Negative
  20-30 Units: Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen
  31 Units or greater: Positive - Suggestive of autoimmune hepatitis type 1 or chronic active hepatitis

  Interpretive Data:
  F-actin antibodies have been shown to have greater sensitivity and specificity for autoimmune liver disease than anti-smooth muscle antibodies.

• Day Run:
  Sun-Sat (daily)
• Time Reported:
  1-2 days
• Test Type:
  IMMUNOLOGY