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Test Name:
Bence Jones Protein, Quantitative Free Kappa and Lambda Light Chains, Urine

• SBMF No:
  45515
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  BJ QUANT-
• ABN:
  Not required
• CPT Code:
  84156; 86335; 83883x2
• LOINC Code:
  29947-9; 30211-7; 19153-6; 2889-4; 29946-1; 29945-3; 29951-1; 29899-2; 13440-3; 38176-4; 38177-2; 38178-0; 38169-9; 41759-2
• Ref Lab Test No:
  0050618
• Test Includes:
  Total Protein, Urine
  Albumin, Urine
  Alpha-1 Globulins, Urine
  Alpha-2 Globulins, Urine
  Beta Globulins, Urine
  Gamma, Urine
  Free Urinary Kappa Light Chains
  Free Urinary Kappa Excretion/Day
  Free Urinary Lambda Light Chains
  Free Urinary Lambda Excretion/Day
  Free Urinary Kappa/Lambda Ratio
  IFE Interpretation
• Also Known As:
  Free Kappa and Lambda Light Chains (Bence Jones Protein), Urine by Immunofixation Electrophoresis, Quantitative
• Also See:
  23055 Electrophoresis, Protein, with Immunofixation, Urine, 24-Hour
  23219 Kappa/Lambda Free Light Chains, Quantitative, with Ratio, Urine, 24-Hour
• Spec Type:
  Urine, 24-hour
• Spec Container:
  24-hour urine container (no preservative added)
• Pref Vol:
  8.0 mL
• Min Vol:
  4.0 mL
• Spec Collect:
  To begin collection of timed specimen, have patient urinate, noting date and exact time
  Discard this first portion of urine, but document date and time of voiding on container label and test requisition as "start" of timed specimen
  Save all urine voided during designated time period, including portion voided at exact end of time period
  IMPORTANT: Refrigerate entire specimen (2-8°C) during and after collection to prevent bacterial growth
  Document date and time of final voiding on label and requisition as "end" of timed specimen
• Spec Process:
  Upon completion of collection, mix entire specimen well and measure 24-hour volume
  Pour off 8.0 mL of urine into plastic transport tube(s) (Min: 4.0 mL)
  Send urine aliquot with notation of 24-hour urine volume on aliquot label and on requisition
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  2 hours room temperature (20-30°C)
  1 week refrigerated (2-8°C)
  1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Sample not refrigerated
• Spec Remarks:
  Keep refrigerated at all times
  Random urine specimens are acceptable, but not preferred
  Urine supernate is also acceptable
• Methodology:
  Immunofixation Electrophoresis
  Nephelometry
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  Total Protein, Urine: 10-140 mg/d
  Albumin, Urine: Detected
  Alpha-1 Globulins, Urine: None detected
  Alpha-2 Globulins, Urine: None detected
  Beta Globulins, Urine: None detected
  Gamma, Urine: None detected
  Free Urinary kappa Light Chains 0.14 - 2.42 mg/dL
  Free Urinary Kappa Excretion/Day: By report
  Free Urinary Lambda Light Chains: 0.02 - 0.67 mg/dL
  Free Urinary Lambda Excretion/Day: By report
  Free Urinary Kappa/Lambda Ratio 2.04 - 10.37 (ratio)
  IFE Interpretation: By report

  Interpretive Data:
  Total urinary protein is determined nephelometrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimate urinary light chains.

• Day Run:
  Mon-Fri
• Time Reported:
  2-6 days
• Test Type:
  URINE