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Test Name:
Benzodiazepines, by LC-MS/MS, Serum or Plasma


  • SBMF No:
    45458
  • Performance Lab Name:
    ARUP Laboratories
  • Test Mnemonic:
    BENZ SP
  • ABN:
    Not required
  • CPT Code:
    80154
  • LOINC Code:
    42586-8
  • Ref Lab Test No:
    0093300
  • Also Known As:
    7-aminoclonazepam, Alpha-hydroxyalprazolam, Alpha-hydroxytriazolam, Alprax, Alprazolam, Ansilan, Apo-Triazo, Ativan, Centrax, Chlorazepate, Chlordiazepoxide, Clonazepam, Clonopin, Dalmadorm, Dalmane, Demetrin, Desalkylflurazepam, Desmethyldiazepam, Desoxydemoxepam, Diastat, Diazepam, Dormicum, Halcion, Hydroxyethylflurazepam, Hypnovel, Klonopin, Librium, Lorazepam, Lysanxia, Medazepam, Midazolam, Mono Demetrin, Niravam, Nobrium, Nordazepam, Nordiazepam, Novo-Clopate, Oxazepam, Paxipam, Pozapam, Prasepine, Prazene, Prazepam, Ravotril, Reapam, Restoril, Rudotel, Serax, Temazepam, Temesta, Tranxene, Trepidan, Valium, Valrelease, Versed, Xanax, Xanor
  • Also See:
    44485 Alprazolam, Serum or Plasma
    44018 Clonazepam, Serum or Plasma
    44964 Diazepam and Nordiazepam, Serum or Plasma
    44541 Lorazepam, Serum or Plasma
  • Spec Type:
    Plasma
  • Spec Container:
    Two (2) Gray top (fluoride/oxalate), green top (sodium heparin), or lavender top (EDTA) tubes
  • Alt Spec Type:
    Serum
  • Alt Spec Container:
    Two (2) Red top (serum) tubes
  • Pref Vol:
    4.0 mL
  • Min Vol:
    2.0 mL
  • Fasting:
    No
  • Spec Collect:
    Do not use gel-barrier tubes for specimen collection
    Routine venipuncture
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    After separation from cells:
    1 week room temperature (20-30°C)
    2 weeks refrigerated (2-8°C)
    3 years frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Sample exposed to repeated freeze/thaw cycles
    Sample collected in gel-barrier tube
    Sample collected in light blue top (sodium citrate) tube
  • Spec Remarks:
    Remove serum/plasma from cells ASAP
  • Methodology:
    Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  • Reference Range:

    Interpretive Data:
    Drugs covered:     alprazolam, alpha-hydroxyalprazolam, clonazepam, 7-amino-clonazepam, desalkylflurazepam, 2-hydroxyethylflurazepam, diazepam, lorazepam, midazolam, nordiazepam, oxazepam, temazepam, and alpha-hydroxytriazolam.

    Identification of specific drug(s) taken by specimen donor is problematic due to common metabolites, some of which are prescription drugs themselves.

    Positive cutoff: 20 ng/mL

    The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

    For medical purposes only; not valid for forensic use.

  • Day Run:
    Sun-Sat (daily)
  • Time Reported:
    2-5 days
  • Test Type:
    TOX-TDM

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