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Test Name:
Platelet Antigen 3 Genotyping (HPA-3)
- SBMF No:
45435 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
HPA3 - ABN:
Required – Not FDA-Approved - CPT Code:
83891; 83896x2; 83898; 83912 - Ref Lab Test No:
0051311 - Also See:
45432 Platelet Antigen Genotyping Panel - Spec Type:
Whole blood - Spec Container:
Lavender top (EDTA) or Pale Yellow top (ACD-A or B) tube - Alt Spec Type:
Amniotic fluid or Cultured amniocytes - Alt Spec Container:
Amniotic fluid: Sterile plastic tube with screw-top cap
Cultured amniocytes: Two T-25 Flasks - Pref Vol:
Whole blood: 3.0 mL
Amniotic fluid: 10.0 mL - Min Vol:
Whole blood: 1.0 mL
Amniotic fluid: 5.0 mL - Fasting:
No - Patient Prep:
Counseling and informed consent are recommended for genetic testing
Forms required:
Informed Consent for Molecular Genetic Testing
Patient History for Molecular Genetic Testing - Spec Collect:
Blood: Routine venipuncture
Amniotic fluid: Collected by physician - Spec Process:
Whole blood:
Gently invert tube to mix blood with the anticoagulant
Do not centrifuge
Do not remove plasma from cells
Forward entire tube of unprocessed whole blood to the laboratoryAmniotic fluid:
Do not centrifugeCultured amniocytes:
Fill flasks with culture media
Transport two T-25 flasks at 80% confluency of cultured amniocytes
Backup cultures must be retained at the client's institution until testing is complete
If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services - Spec Store Transport:
Whole blood: Room temperature (72 hours) or refrigerated
Cultured cells or amniotic fluid: Room temperature - Spec Stability:
Whole blood:
72 hours room temperature (20-30°C)
1 week refrigerated (2-8°C)
Frozen (-20°C): UnacceptableAmniotic fluid or cultured amniocytes:
48 hours room temperature (20-30°C)
Refrigerated (2-8°C): Unacceptable
Frozen (-20°C): Unacceptable - Spec Remarks:
Cultured Amniocytes: CRITICAL ROOM TEMPERATURE – Must be received at ARUP within 48 hours of shipment due to lability of cells - Methodology:
Polymerase Chain Reaction (PCR)
Fluorescence Monitoring - Clinical Significance:
Refer to:
- Reference Range:
By report
Interpretive Data:
Background Information for Platelet Antigen 3 Genotyping (HPA-3):
Characteristics: Spontaneous fetal intracranial bleeding in 20 percent of severe PAT affected pregnancies; risk of fetal death. Post-transfusion purpura in transfusion recipients with antibodies to a specific platelet antigen.
Incidence: Perinatal alloimmune thrombocytopenia (PAT) occurs in 1 in 5000 births.
Inheritance: For women homozygous for a rare b HPA allele with antibodies to the common a allele, there is a 50 percent risk a pregnancy will be affected if her partner is heterozygous for a allele and 100 percent risk if her partner is homozygous for a allele.
Mutations Tested: HPA-3 a (common) and b (variant) alleles.
Limitations: Mutations other than the one examined will not be detected; furthermore, mutations within the primer or probe regions could affect this assay.
Sensitivity: 99 percentThis test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
- Additional Test Info:
Genetic Compliance Statement:
The performance characteristics of this test were validated by ARUP Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test. However, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. ARUP is authorized under Clinical Laboratory Improvement Amendments (CLIA) and by all states to perform high-complexity testing.Counseling and informed consent are recommended for genetic testing. Consent forms are available online.
- Day Run:
Mon, Thu - Time Reported:
3-8 days - Test Type:
GENETIC
