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Test Name:
Euglobulin Clot Lysis Time
- SBMF No:
45383 - Performance Lab Name:
Quest Diagnostics Nichols Institute - Test Mnemonic:
ECLT - ABN:
Not required - CPT Code:
85360 - Ref Lab Test No:
462N - Also Known As:
ECLT - Spec Type:
Platelet-poor plasma (PPP) - Spec Container:
Light Blue top (3.2% buffered sodium citrate) tube - Pref Vol:
2.0 mL - Min Vol:
1.0 mL - Fasting:
No - Patient Prep:
Prohibit exercise prior to drawing sample - Spec Collect:
- Routine venipuncture
- • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
• Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
• Fill light blue top tube to stated tube volume
• If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
• If multiple coagulation studies are requested, submit additional samples for each coagulation test
• Immediately after collection, gently invert tube to mix
- Spec Process:
- Separate plasma from cells immediately
- • Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• NOTE: Buffy coat layer of sample must be avoided
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
• Immediately freeze PPP samples
- Spec Store Transport:
Store Deep-frozen (-70°C)
Transport frozen on dry ice - Spec Stability:
Room temperature (20-30°C): Unacceptable
4 hours refrigerated (2-8°C)
3 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Thawed sample - Methodology:
Clot Dissolution - Clinical Significance:
ECLT provides an overall assessment of the fibrinolysis system by measuring the time for an in vitro clot to dissolve in the absence of the normal plasmin inhibitors.
ECLT is useful in assessing the fibrinolytic system and monitoring patients on urokinase or streptokinase fibrinolytic therapy.
- Reference Range:
> 60 min - Day Run:
Varies - Time Reported:
Varies - Test Type:
COAGULATION
