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Test Name:
Cell-Mediated Immune Function Screen

• SBMF No:
  45367
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  LMPS
• ABN:
  Not required
• CPT Code:
  86352
• LOINC Code:
  19146-0
• Ref Lab Test No:
  0051273
• Also Known As:
  ImmuKnow™ Immune Cell Function Assay
  Immune Cell Function Assay
• Also See:
  45368 Transplantation Immune Cell Function Assay
• Spec Type:
  Whole blood in original collection tube
• Spec Container:
  Green top (sodium heparin) tube
• Pref Vol:
  3.0 mL
• Min Vol:
  0.5 mL
• Fasting:
  No
• Spec Collect:
  Do not collect on or immediately prior to a weekend or holiday
  Sample must be collected within 30 hours of test performance
  Routine venipuncture
• Spec Process:
  Gently invert tube to mix
  Do not centrifuge
  Do not remove plasma from cells
  Do not transfer sample from original collection tube
  Sample cannot be refrigerated or frozen
• Spec Store Transport:
  Room temperature
  Time-sensitive – Sample must be collected within 30 hours of test performance
  Remote clients may call Client Services to arrange direct referral to performing lab
• Spec Stability:
  30 hours room temperature (20-30°C)
  Refrigerated (2-8°C): Unacceptable
  Frozen (-20°C): Unacceptable
• Spec Reject:
  Refrigerated or frozen sample
  Sample in transport longer than 30 hours
• Spec Remarks:
  CRITICAL ROOM TEMPERATURE. Do not refrigerate or freeze.
  LIVE LYMPHOCYTES REQUIRED
• Methodology:
  Cell Culture
  Quantitative Chemiluminescence
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  226-524 ng/mL

  Interpretive Data:
  This assay quantifies the concentration of adenosine triphosphate (ATP) produced by circulating immune cells in response to phytohemagglutinin-L (PHA-L) stimulation. This assay does not directly quantify the level of immunosuppression.

  ATP Level	Result	Interpretation
  225 ng/mL or    lower	Low	The patient's circulating immune cells are showing    low response to PHA-L stimulation.
  226-524 ng/mL	Moderate	The patient's circulating immune cells are showing moderate response to PHA-L stimulation.
  525 ng/mL or greater	Strong	The patient's circulating immune cells are showing strong response to PHA-L stimulation.

• Additional Test Info:
  This assay is performed using the ImmunKnow™ Immune Cell Function Assay.
• Day Run:
  Mon-Sun
• Time Reported:
  2-3 days
• Test Type:
  IMMUNOLOGY