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Test Name:
Fentanyl and Metabolite, Serum or Plasma


  • SBMF No:
    45260
  • Performance Lab Name:
    ARUP Laboratories
  • Test Mnemonic:
    FENTANYLSP
  • ABN:
    Not required
  • CPT Code:
    83925
  • LOINC Code:
    19146-0
  • Ref Lab Test No:
    0092569
  • Test Includes:
    Fentanyl
    Norfentanyl (Metabolite)
  • Also Known As:
    Sublimaze®
  • Spec Type:
    Plasma
  • Spec Container:
    Gray top (fluoride/oxalate) tube
  • Alt Spec Type:
    Serum or plasma
  • Alt Spec Container:
    Red top (serum) tube, green top (sodium heparin) tube, or lavender top (EDTA) tube
  • Pref Vol:
    4.0 mL
  • Min Vol:
    2.5 mL
    Pediatric Collection: 1.5 mL
  • Fasting:
    No
  • Spec Collect:
    Do not use gel-barrier tubes for specimen collection
    Routine venipuncture
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube
  • Spec Store Transport:
    Room temperature
  • Spec Stability:
    After separation from cells:
    1 month room temperature (20-30°C)
    1 month refrigerated (2-8°C)
    3 years frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Sample exposed to repeated freeze/thaw cycles
    Sample collected in gel-barrier tube or lt. blue top (sodium citrate) tube
  • Spec Remarks:
    Separate serum or plasma from cells ASAP
  • Methodology:
    Liquid Chromatography-Tandem Mass Spectrometry
  • Clinical Significance:
    Refer to:
    ARUP Consult | The Physician's Guide to Laboratory Test Selection and Interpretation
  • Reference Range:
    Reference Interval:
    By report
    Interpretive Data:
    For medical purposes only; not valid for forensic use
    Drugs covered: fentanyl and metabolite (norfentanyl)
    Positive cutoff: 0.1 ng/mL
  • Day Run:
    Sun-Sat (daily)
  • Time Reported:
    2-5 days
  • Test Type:
    TOX-TDM