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Test Name:
Barbiturates, by GC/MS, Serum or Plasma


  • SBMF No:
    44961
  • Performance Lab Name:
    ARUP Laboratories
  • Test Mnemonic:
    BARB SP
  • ABN:
    Not required
  • CPT Code:
    82205
  • Ref Lab Test No:
    0093308
  • Also Known As:
    Amobarbital
    Amobarbitone
    Amytal
    Axocet
    Butalbital
    Fioricet
    Fiorinal
    Luminal
    Nembutal
    Pentobarbital
    Phenobarbital
    Phenobarbitone
    Quinalbartbitone
    Sandoptal
    Secobarbital
    Seconal
    Solfoton
    Tuinal
  • Spec Type:
    Plasma
  • Spec Container:
    Gray top (fluoride/oxalate), green top (sodium heparin), or lavender top (EDTA) tube
  • Alt Spec Type:
    Serum
  • Alt Spec Container:
    Red top (serum) tube
  • Pref Vol:
    4.0 mL
  • Min Vol:
    2.0 mL
  • Fasting:
    No
  • Spec Collect:
    Do not use gel-barrier tubes for specimen collection
    Routine venipuncture
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week room temperature (20-30°C)
    2 weeks refrigerated (2-8°C)
    3 years frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Sample exposed to repeated freeze/thaw cycles
    Sample collected in gel-barrier tube
    Sample collected in light blue top (sodium citrate) tube
  • Spec Remarks:
    Remove serum/plasma from cells ASAP
  • Methodology:
    Gas Chromatography-Mass Spectrometry (GC/MS)
  • Reference Range:
    By report

    Interpretive Data:
    Drugs covered:
    butalbital, amobarbital, pentobarbital, secobarbital, and phenobarbital.
    Positive cutoff: 50 ng/mL

    The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

    For medical purposes only; not valid for forensic use.

  • Day Run:
    Sun-Sat (daily)
  • Time Reported:
    2-5 days
  • Test Type:
    TOX-TDM