South Bend Medical Foundation - Your Community Laboratory

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Test Name:
Carotenes, Fractionated, Serum or Plasma

• SBMF No:
  44920
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  CARO FRAC
• ABN:
  Not required
• CPT Code:
  82380
• LOINC Code:
  56669-5; 19146-0; 2053-7; 19146-0
• Ref Lab Test No:
  0021021
• Test Includes:
  Alpha-carotene
  Beta-carotene
  Lutein
  Zeaxanthin
• Also See:
  23034 Carotene, Total, Serum
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Alt Spec Type:
  Plasma
• Alt Spec Container:
  Green top (heparin) tube
• Pref Vol:
  1.0 mL
• Min Vol:
  0.3 mL
• Fasting:
  Yes
• Patient Prep:
  Patient should fast overnight for 12 hours and should not consume alcohol for 24 hours prior to blood draw
• Spec Collect:
  Routine venipuncture
  Protect from light
• Spec Process:
  Serum sample tubes, clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum or plasma to light-protected (amber) plastic tube and freeze
• Spec Store Transport:
  Frozen
  Protect from light
• Spec Stability:
  After separation from cells:
  Room temperature (20-30°C): Unacceptable
  72 hours refrigerated (2-8°C)
  1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Room temperature sample
  EDTA plasma, whole blood, or body fluid sample
  Hemolyzed sample
• Spec Remarks:
  CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered
  Protect from light within one hour of collection and during storage and shipment
  Separate serum or plasma from cells ASAP or within 2 hours of collection
• Methodology:
  Quantitative High Performance Liquid Chromatography (HPLC)
• Clinical Significance:
  Refer to:
  
• Reference Range:
  α-Carotene: 9-101 µg/L
  Β-Carotene: 42-373 µg/L
  Lutein: 50-250 µg/L
  Zeaxanthin: 8-80 µg/L

  Interpretive Data:
  Carotenoid concentrations may be decreased due to inadequate dietary fat consumption, intestinal parasitic infection or other malabsorption disorders; interactions from drugs such as cholestyramine, ethanol, weight-loss drugs, and fiber supplements; and kidney, liver, or pancreatic insufficiency. Carotenoid concentrations may be elevated due to hypothyroidism, hyperlipemia due to diabetes mellitus, myxedema, chronic nephritis, or vitamin supplementation.

• Day Run:
  Mon, Wed, Fri
• Time Reported:
  2-6 days
• Test Type:
  CHEMISTRY