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Test Name:
Tissue Plasminogen Activator Antigen
- SBMF No:
44866 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
TPA AG - ABN:
Not required - CPT Code:
85415 - LOINC Code:
5971-7 - Ref Lab Test No:
0099187 - Also Known As:
tPA Ag
tPA Antigen - Spec Type:
Platelet-poor plasma (PPP) - Spec Container:
Light blue top (3.2% buffered sodium citrate) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
- Routine venipuncture
- • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
• Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
• Fill light blue top tube to stated tube volume
• If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
• If multiple coagulation studies are requested, submit additional samples for each coagulation test
• Immediately after collection, gently invert tube to mix
- Spec Process:
- Separate plasma from cells immediately
- • Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• NOTE: Buffy coat layer of sample must be avoided
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
• Immediately freeze PPP samples
- Spec Store Transport:
Frozen - Spec Stability:
4 hours room temperature (20-30°C)
4 hours refrigerated (2-8°C)
2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Serum sample
Sample collected in anticoagulant other than sodium citrate - Spec Remarks:
Separate plasma from cells and freeze ASAP - Methodology:
Enzyme-Linked Immunosorbent Assay (ELISA) - Clinical Significance:
Refer to:
- Reference Range:
0.8-11.2 ng/mL - Day Run:
Tue, Fri - Time Reported:
2-9 days - Test Type:
COAGULATION
