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Test Name:
Galactose-1-Phosphate in Red Blood Cells

• SBMF No:
  44738
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  GAL1P RBC
• ABN:
  Not required
• CPT Code:
  84378
• Ref Lab Test No:
  0081296
• Also Known As:
  Gal1P
  Galactose 1
  Galactose 1 phosphate
  Galactosemia analyte
  Galactosemia metabolite (or Galactosemia metabolites)
  Galactosemia monitoring
  Galactosemia therapy
  GALT analyte
  GALT monitoring
• Also See:
  45529 Galactose-1-Phosphate Uridyltransferase
  44922 Galactosemia, (GALT) 9 Mutations
  44923 Galactosemia, (GALT) Enzyme Activity and 9 Mutations
• Spec Type:
  Whole blood
• Spec Container:
  Lavender top (EDTA) or green top (heparin) tube
  Collect on ice
• Alt Spec Type:
  Washed red blood cells (see Specimen Remarks below)
• Alt Spec Container:
  Lavender top (EDTA) or green top (heparin) tube
  Collect on ice
• Pref Vol:
  5.0 mL
• Min Vol:
  2.0 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  Gently invert tube to mix
  Place tube on wet ice immediately after collection
  Do not centrifuge
  Do not remove plasma from cells
  Specimen cannot be frozen
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  Room temperature (20-30°C): Unacceptable
  3 days refrigerated (2-8°C)
  Frozen (-20°C): Unacceptable
• Spec Reject:
  Room temperature or frozen whole blood sample
• Spec Remarks:
  

  Alternate acceptable specimen is frozen, washed red blood cells.
  1. Centrifuge whole blood immediately for 10 minutes at 800 g RCF.
  2. Discard the plasma and buffy coat layers.
  3. Add cold 0.9 percent saline solution to the red cells (about 2 times the volume of cells) and mix gently by inverting the tube.
  4. Centrifuge for 10 minutes at 800 g RCF.
  5. Aspirate and discard the supernatant.
  6. Repeat the wash (steps 3 through 5) 2 more times.
  7. After the 3rd wash and centrifugation, remove the supernatant and a thin layer of the top cells.
  8. Transfer washed cells to an ARUP standard transport tube freeze. Ship on dry ice and indicate on sample and/or requisition that washed red cells have been submitted.

  Washed red cells must be frozen. Ambient and refrigerated washed cells are unacceptable.

• Methodology:
  Gas Chromatography-Mass Spectrometry (GC/MS)
• Clinical Significance:
  Refer to:
  
• Reference Range:
  

  Galactose-1-phosphate
  0.0-1.0 mg/dL

  Galactose-1-phosphate (ug/g Hb)
  0-53 ug/g Hb

  Galactose-1-phosphate (umol/g Hb)
  0.00-0.20 umol/g Hb

• Day Run:
  Tue
• Time Reported:
  3-10 days
• Test Type:
  CHEMISTRY