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Test Name:
Reptilase Time with Reptilase Time 1:1 Mix if Indicated


  • SBMF No:
    44671
  • Performance Lab Name:
    ARUP Laboratories
  • Test Mnemonic:
    REP
  • ABN:
    Not required
  • CPT Code:
    85635
    If indicated, add 85635
  • LOINC Code:
    6683-7; 33526-5
  • Ref Lab Test No:
    0030295
  • Test Includes:
    Reptilase Time
    Reptilase Time 1:1 Mix (if indicated)
  • Spec Type:
    Platelet-poor plasma (PPP)
  • Spec Container:
    Light Blue top (3.2% buffered sodium citrate) tube
  • Pref Vol:
    2.0 mL
  • Min Vol:
    1.0 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
    • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
    • Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
    • Fill light blue top tube to stated tube volume
    • If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
    • If multiple coagulation studies are requested, submit additional samples for each coagulation test
    • Immediately after collection, gently invert tube to mix
  • Spec Process:
    Separate plasma from cells immediately
    • Promptly centrifuge 15 minutes
    • Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
    • NOTE: Buffy coat layer of sample must be avoided
    • Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
    • Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
    • Immediately freeze PPP samples
  • Spec Store Transport:
    Frozen
  • Spec Stability:
    2 hours room temperature (20-30°C)
    4 hours refrigerated (2-8°C)
    2 weeks frozen (-20C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Serum sample
    Nonfrozen or hemolyzed samples
  • Spec Remarks:
    CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered
  • Methodology:
    Clotting
  • Reference Range:

    Reptilase Time
    Less than 22.0 seconds

    Reptilase Time 1:1 Mix
    Less than 22.0 seconds

    Note:
    If Reptilase Time is elevated, then Reptilase Time 1:1 mix will be added. Additional charges apply.

  • Day Run:
    Mon-Sat
  • Time Reported:
    2-4 days
  • Test Type:
    COAGULATION

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