South Bend Medical Foundation - Your Community Laboratory

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Test Name:
Brucella Total Antibody, by Agglutination

• SBMF No:
  44605
• Performance Lab Name:
  ARUP Laboratories
• Test Mnemonic:
  BRUC
• ABN:
  Not required
• CPT Code:
  86622
• LOINC Code:
  19053-8
• Ref Lab Test No:
  0050135
• Also Known As:
  Brucella Antibody (Total) by Agglutination
• Also See:
  33060 Brucella IgG and IgM Antibodies, by ELISA
  45389 Febrile Antibodies Panel
  45178 Francisella tularensis Total Antibodies
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  1.0 mL
• Min Vol:
  0.2 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
  Parallel testing is preferred
  Label samples as: "acute" or "convalescent"
  Convalescent samples must be received within 30 days from receipt of acute samples
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  After separation from cells:
  48 hours room temperature (20-30°C)
  2 weeks refrigerated (2-8°C)
  1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Severely lipemic, contaminated, heat-inactivated, or hemolyzed sample
• Spec Remarks:
  Separate serum from cells ASAP
• Methodology:
  Bacterial Agglutination
• Clinical Significance:
  Refer to:
  
• Reference Range:
  < 1:20 Negative

  Interpretive Data:
  Cross-reactions may occur between Brucella and F. tularensis antigens and antisera, therefore, parallel tests should be run with these antigens. A fourfold rise in titer is considered diagnostic. A single serum titer of 1:80 or 1:160 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.

• Day Run:
  Mon-Fri
• Time Reported:
  3-5 days
• Test Type:
  INFECTIOUS ANTIBODY