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Test Name:
Nicotine and Metabolites, Serum or Plasma
- SBMF No:
44552 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
NICOTINE - ABN:
Not required - CPT Code:
83887 - LOINC Code:
3853-9; 10365-5; 35140-3 - Ref Lab Test No:
0092361 - Test Includes:
Nicotine
Cotinine
3-OH-Cotinine - Spec Type:
Serum - Spec Container:
Red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Green top (sodium heparin) or lavender top (EDTA) tube - Pref Vol:
4.0 mL - Min Vol:
2.0 mL - Fasting:
No - Spec Collect:
Do not use gel-barrier tubes for specimen collection
Routine venipuncture - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube - Spec Store Transport:
Refrigerated - Spec Stability:
After separation from cells:
1 week room temperature (20-30°C)
2 weeks refrigerated (2-8°C)
3 years frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Sample exposed to repeated freeze/thaw cycles
Sample collected in gel-barrier tube or lt. blue top (sodium citrate) tube - Spec Remarks:
Separate serum or plasma from cells ASAP - Methodology:
Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS) - Clinical Significance:
Refer to:
- Reference Range:
- Unexposed non-tobacco user
- Nicotine: Less than 2 ng/mL
Cotinine: Less than 2 ng/mL
3-OH-Cotinine: Less than 2 ng/mL - Passive exposure
- Nicotine: Less than 2 ng/mL
Cotinine: Less than 8 ng/mL
3-OH-Cotinine: Less than 2 ng/mL - Abstinent user for greater than 2 weeks
- Nicotine: Less than 2 ng/mL
Cotinine: Less than 2 ng/mL
3-OH-Cotinine: Less than 2 ng/mL - Active tobacco product use
- Nicotine: 30-50 ng/mL
Cotinine: 200-800 ng/mL
3-OH-Cotinine: 100-500 ng/mL
Adapted from Clinical Chemistry 48:9 1460-1471, 2002
Interpretive Data:- Drugs covered: nicotine, cotinine, 3-OH-cotinine.
Positive cutoff: 2 ng/mL
The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.
For medical purposes only; not valid for forensic use.
- Day Run:
Sun-Sat (daily) - Time Reported:
2-5 days - Test Type:
TOX-TDM
