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Test Name:
Vitamin C (Ascorbic Acid)
- SBMF No:
44550 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
VIT-C - ABN:
Not required - CPT Code:
82180 - LOINC Code:
1903-4 - Ref Lab Test No:
0080380 - Also Known As:
Ascorbic Acid - Spec Type:
Plasma - Spec Container:
Two (2) Green top (heparin) tubes, AND
Two (2) special collection tubes containing 40 mg oxalic acid - Alt Spec Type:
Plasma - Alt Spec Container:
Two (2) Lavender top (EDTA) tubes, AND
Two (2) special collection tubes containing 40 mg oxalic acid - Pref Vol:
4.0 mL oxalic acid-preserved plasma - Min Vol:
1.3 mL oxalic acid-preserved plasma - Fasting:
Preferred - Spec Collect:
Routine venipuncture - Spec Process:
Promptly centrifuge 15 minutes
Immediately transfer 2.0 mL plasma to each of 2 separate plastic collection tubes containing 40.0 mg oxalic acid
Mix and freeze
Adequate preservation is 20 mg oxalic acid to 1 mL plasma - Spec Store Transport:
Frozen - Spec Stability:
After separation from cells:
Room temperature (20-30°C): Unacceptable
Refrigerated (2-8°C): Unacceptable
2 months frozen (-20°C) (with oxalic acid preservative added) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Serum and hemolyzed plasma samples
Nonfrozen samples
Samples that are not preserved with oxalic acid - Spec Remarks:
CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered
The oxalic acid preservative may interfere with other tests
Special collection tubes are available through Client Services - Methodology:
Spectrophotometry (DNPH) - Clinical Significance:
Refer to:
- Reference Range:
0.4-2.0 mg/dL
Interpretive Data:
Vitamin C concentrations between 0.2 and 0.4 mg/dL indicate risk of deficiency. Concentrations less than 0.2 mg/dL are consistent with deficiency. - Day Run:
Sun, Tue, Thu - Time Reported:
2-8 days - Test Type:
CHEMISTRY
