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Test Name:
Epithelial Skin Antibody

• SBMF No:
  44520
• Performance Lab Name:
  University of Utah Health Sciences Center Immunodermatology Laboratory
• Test Mnemonic:
  EPITHELIAL
• ABN:
  Not required
• CPT Code:
  88347x4
• Ref Lab Test No:
  ARUP# 0090299
• Test Includes:
  Basement Membrane Zone (MBZ) IgG & IGA Antibodies, by IIF
  Cell Surface IgG & IGA Antibodies, by IIF
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  2.0 mL
• Min Vol:
  0.5 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
• Spec Store Transport:
  Room temperature (4 days) or refrigerated
• Spec Stability:
  4 days room temperature (20-30°C)
  3 months refrigerated (-2-8°C)
  Frozen (-20°C): Indefinitely – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Hemolyzed or lipemic sample
• Methodology:
  Indirect Immunofluorescence
• Use:
  Monitor treatment response
  Use along with endomysial antibody, IgA, IgG desmoglein-1 and IgG desmoglein-3 antibodies and IgG BP180 and BP230 antibodies tests to differentiate among the immunobullous skin diseases in patients suspected or known to have these disorders
• Clinical Significance:
  

  Basement membrane zone antibodies by Indirect Immunofluorescence (IIF) of serum
  The IIF test is performed on serum to detect the presence and amount of circulating IgG and/or IgA antibodies to epidermal/epithelial antigens. Semiquantitative levels of antibodies are reported as titers. Basement membrane zone antibodies are helpful in diagnosing and distinguishing subepidermal immunobullous diseases such as pemphigoid, epidermolysis bullosa acquisita and linear IgA bullous disease.

  Cell surface (also known as intercellular substance and pemphigus) antibodies by Indirect Immunofluorescence (IIF) of serum
  The IIF test is performed on serum to detect the presence and amount of circulating IgG and/or IgA antibodies to epidermal/epithelial antigens. Semiquantitative levels of antibodies are reported as titers. Cell surface antibodies are helpful in diagnosing pemphigus, including pemphigus vulgaris, pemphigus foliaceus, pemphigus vegetans and pemphigus erythematosus for IgG cell surface antibodies and IgA pemphigus for IgA cell surface antibodies. Levels of antibodies correlate with disease activity in pemphigus.

  Also see:
  

• Reference Range:
  By report
• Additional Test Info:
  This test is referred through ARUP Laboratories
• Day Run:
  Varies
• Time Reported:
  5-10 days
• Test Type:
  IMMUNOLOGY