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Test Name:
Platelet Antibodies, Indirect
- SBMF No:
44477 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
PLT ABSCRN - ABN:
Not required - CPT Code:
86022 - LOINC Code:
24375-8 - Ref Lab Test No:
0051050 - Also Known As:
Antiplatelet, Circulating Platelet Antibodies
Platelet Antibodies, Qualitative - Also See:
45459 Platelet Antibody Identification
44007 Platelet Associated Antibodies, Direct Assay - Spec Type:
Serum - Spec Container:
Red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Lavender top (EDTA) tube or pale yellow top (ACD-A) tube - Pref Vol:
1.0 mL - Min Vol:
Pediatric Collection: 0.5 mL - Fasting:
No - Spec Collect:
Do not use gel-barrier tubes for specimen collection
Routine venipuncture
Note: For newborns less than 30 days old, collect sample from mother - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube and freeze - Spec Store Transport:
Frozen - Spec Stability:
After separation from cells:
Room temperature (20-30°C): Unacceptable
2 days refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Microbially contaminated, hemolyzed, lipemic, or heat-inactivated sample - Spec Remarks:
Separate serum or plasma from cells ASAP and freeze
Frozen serum or plasma is the specimen of choice, however, specimens that have been maintained at 2-8°C for 48 hours or less will be accepted - Methodology:
Enzyme-Linked Immunosorbent Assay (ELISA) - Reference Range:
None detected
Interpretive Data:
This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodies, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions.This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [PlA1 and PlA2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class I antigens (HLA-A-B).
Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples.
Note:
Further characterization of antibodies directed to platelet glycoproteins IIb/IIIa may be performed at client request by ordering Platelet Antibody Identification (45459). - Day Run:
Mon-Sat - Time Reported:
2-4 days - Test Type:
IMMUNOLOGY
