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Test Name:
Fatty Acids, Free, Serum or Plasma
- SBMF No:
44390 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
FFA - ABN:
Not required - CPT Code:
82725 - LOINC Code:
15066-4 - Ref Lab Test No:
0080120 - Also Known As:
Free Fatty Acids - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Lavender top (EDTA), light blue top (sodium citrate), or gray top (fluoride/oxalate) tube - Pref Vol:
1.0 mL - Min Vol:
0.2 mL - Fasting:
Yes - Patient Prep:
Overnight fasting sample is preferred - Spec Collect:
Routine venipuncture
Collect on ice - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube and freeze - Spec Store Transport:
Frozen - Spec Stability:
After separation from cells:
Room temperature (20-30°C): Unacceptable
4 hours refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Non-frozen or heparinized sample - Spec Remarks:
CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered
Serum or plasma must be separated from cells and frozen ASAP, otherwise, lipase continues to break down triglycerides, giving rise to elevated levels of nonesterified (free) fatty acids - Methodology:
Spectrophotometry - Clinical Significance:
Refer to:
- Reference Range:
0-30 days: 0.43-1.37 mmol/L
1 month-9 years: 0.50-0.90 mmol/L
10 years and older: 0.10-0.60 mmol/L - Day Run:
Mon, Wed, Fri - Time Reported:
2-5 days - Test Type:
CHEMISTRY
