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Test Name:
Neutrophil Oxidative Burst Assay (DHR)
- SBMF No:
44374 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
DHR - ABN:
Required – Not FDA-Approved - CPT Code:
86352 - LOINC Code:
19146-0 - Ref Lab Test No:
0096657 - Also Known As:
Chronic granulomatous disease (CGD)
DHR
Nitroblue Tetrazolium (NBT) - Spec Type:
Whole blood from both the patient and a normal control subject - Spec Container:
Green top (sodium heparin) tube - Pref Vol:
3.0 mL from patient, AND
3.0 mL from a normal control - Fasting:
No - Spec Collect:
Do not collect samples on a weekend or holiday
Routine venipuncture
Collect samples from patient and normal control subject
Normal control must be a healthy individual who is unrelated to the patient
Patient and control samples must be collected within 48 hours of test performance - Spec Process:
Do not centrifuge
Do not remove plasma from cells
Do not transfer blood to other containers (send in original collection tubes)
Specimen cannot be refrigerated or frozen - Spec Store Transport:
LIVE NEUTROPHILS REQUIRED
CRITICAL AMBIENT (room temperature)
Do not refrigerate or freeze - Spec Stability:
48 hours room temperature (20-30°C)
Refrigerated (2-8°C): Unacceptable
Frozen (-20°C): Unacceptable - Spec Reject:
Refrigerated or frozen samples
Samples in transit longer than 48 hours - Methodology:
Flow Cytometry - Clinical Significance:
Refer to:
- Reference Range:
By report
Interpretive Data:
White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry.Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).
Note:
If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport. For abnormal results, we encourage consultation with the ARUP Immunology Medical Director.Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
- Additional Test Info:
This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. - Day Run:
Sun-Sat (daily) - Time Reported:
3-4 days - Test Type:
FLOW
