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Test Name:
West Nile Virus RNA, by RT-PCR
- SBMF No:
44324 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
WNILE PCR - ABN:
Required – Not FDA-Approved - CPT Code:
87798 - LOINC Code:
19146-0; 32361-8 - Ref Lab Test No:
0050229 - Also Known As:
WNV by PCR - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Cerebrospinal fluid (CSF) - Alt Spec Container:
Sterile plastic tube with screw-top cap - Pref Vol:
1.0 mL - Min Vol:
Pediatric Collection: 0.3 mL
Note: Less volume may decrease sensitivity of assay - Fasting:
No - Spec Collect:
Blood: Routine venipuncture
Cerebrospinal fluid: Collected by physician - Spec Process:
- Serum specimen:
- – Centrifuge serum sample tube
– Immediately transfer serum to separate sterile plastic tube and freeze
– Separate samples must be submitted when multiple tests are ordered - CSF specimen: freeze and transport frozen
Note specimen source on container label and test request form
- Spec Store Transport:
Frozen - Spec Stability:
1 hour room temperature (20-30°C)
24 hours refrigerated (2-8°C)
3 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Heparinized or hemolyzed sample
Non-sterile or leaking container - Methodology:
Reverse Transcription Polymerase Chain Reaction (RT-PCR) - Clinical Significance:
Refer to:
- Reference Range:
- West Nile Virus RNA by RT-PCR
- Negative: West Nile virus nucleic acid not detected by RT-PCR
Positive: West Nile virus nucleic acid detected by RT-PCR - Interpretive Data:
- A negative result does not rule out the presence of PCR inhibitors in the patient specimen of West Nile virus nucleic acid in concentrations below the level of detection of the assay.
- Additional Test Info:
This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
- Day Run:
Mon-Fri - Time Reported:
2-5 days - Test Type:
INFECTIOUS ORGANISM
