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Test Name:
Vitamin B6 (Pyridoxal 5-Phosphate)
- SBMF No:
44322 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
VIT-B6 - ABN:
Not required - CPT Code:
84207 - LOINC Code:
2900-9 - Ref Lab Test No:
0080111 - Also Known As:
B6 Vitamin
Pyridoxal 5-Phosphate
Pyridoxal Phosphate - Spec Type:
Plasma - Spec Container:
Green top (heparin) or light green top (PST) tube - Alt Spec Type:
Serum - Alt Spec Container:
Red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Fasting:
Yes - Patient Prep:
Collect specimen after an overnight fast - Spec Collect:
Routine venipuncture
Protect from light - Spec Process:
Promptly centrifuge 15 minutes
Immediately transfer plasma or serum to separate amber plastic tube and freeze - Spec Store Transport:
Frozen
Protect from light - Spec Stability:
After separation from cells:
Room temperature (20-30°C): Unacceptable
4 hours refrigerated (2-8°C)
2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Any sample other than heparinized plasma or serum
Hemolyzed or non-frozen sample
Sample collected in gold top (SST) tube - Spec Remarks:
CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered
Protect from light during collection, storage, and shipment
Separate plasma or serum from cells and freeze ASAP, or within 2 hours of collection
Specimens collected in EDTA yield a higher pyridoxal 5'-phosphate concentration, therefore EDTA is not acceptable - Methodology:
Quantitative High Performance Liquid Chromatography (HPLC) - Clinical Significance:
Refer to:
- Reference Range:
20-125 nmol/L
Interpretive Data:
Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.Note:
The biologically active form of vitamin B6, pyridoxal 5-phosphate, is measured in this assay. - Day Run:
Sun-Sat (daily) - Time Reported:
2-5 days - Test Type:
CHEMISTRY
