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Test Name:
Entamoeba histolytica IgG Antibody, by ELISA
- SBMF No:
44303 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
AMEB AB - ABN:
Not required - CPT Code:
86753 - LOINC Code:
9522-4 - Ref Lab Test No:
0050070 - Also Known As:
Amebic Antibody
Amebic Serology
Amebiasis
Amoebiasis Antibody
Amoeba Histolytica
Amoeba, Serum
E Histolytica Antibody
Parasite Serology - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL
Pediatric Collection: 0.15 mL - Fasting:
No - Spec Collect:
Routine venipuncture
Parallel testing is preferred
Label samples as: "acute" or "convalescent"
Convalescent samples must be received within 30 days from receipt of acute samples - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube - Spec Store Transport:
Refrigerated - Spec Stability:
After separation from cells:
- 2 days room temperature (20-30°C)
- 2 weeks refrigerated (2-8°C)
- 1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Severely lipemic, contaminated, hemolyzed, or heat-inactivated sample - Methodology:
Enzyme-Linked Immunosorbent Assay (ELISA) - Use:
Confirm past infection
Diagnose invasive extraintestinal disease - Clinical Significance:
Refer to:
- Reference Range:
0.8 IV or less: Negative – No significant level of detectable E. histolytica IgG antibody
0.9-1.1 IV: Equivocal – Repeat testing in 10-14 days may be helpful
1.2 IV or greater: Positive – IgG antibody to E. histolytica detected, suggestive of a current or past infectionSeroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.
- Additional Test Info:
ASR Compliance Statement:
Analyte specific reagents (ASR) are used in many laboratory tests necessary for standard medical care and generally do not require U.S. Food and Drug Administration (FDA) approval or clearance. This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. This test should not be regarded as investigational or for research use. - Day Run:
Tue, Fri - Time Reported:
2-6 days - Test Type:
INFECTIOUS ANTIBODY