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Test Name:
Haemophilus Influenzae b IgG Antibody
- SBMF No:
44096 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
HIBE IGG - ABN:
Not required - CPT Code:
86317 - LOINC Code:
11256-5 - Ref Lab Test No:
0050542 - Also Known As:
H. Influenzae b IgG Antibody
Haemophilus influenzae b Vaccine Response - Also See:
45553 Diphtheria, Tetanus, and H. influenzae b IgG Antibodies - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.15 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube
Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine" - Spec Store Transport:
Refrigerated - Spec Stability:
After separation from cells:
2 days room temperture
2 weeks refrigerated (2-8°C)
1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Plasma or body fluid sample
Contaminated, hemolyzed or, severely lipemic sample - Spec Remarks:
Separate serum from cells ASAP or within 2 hours of collection
"Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing.
"Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. - Methodology:
Quantitative Multi-Analyte Fluorescence Detection (MAFD) - Clinical Significance:
Refer to:
- Reference Range:
< 1.0 µg/mL = Antibody concentration not protective.
≥ 1.0 µg/mL = Antibody to H. influenzae b detected. Suggestive of protection.Interpretive Data:
Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a nonresponder.
2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder. - Day Run:
Sun-Sat - Time Reported:
2-3 days - Test Type:
INFECTIOUS ANTIBODY
