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Test Name:
Western Equine Encephalitis IgG Antibody, by IFA, Serum
- SBMF No:
44061 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
WESTERN - ABN:
Not required - CPT Code:
86654 - LOINC Code:
6957-5 - Ref Lab Test No:
0050514 - Also Known As:
Encephalitis Antibody, Western Equine, IgG
WEE IgG Ab - Also See:
44056 California Encephalitis IgG Antibody, Serum
44059 Eastern Equine Encephalitis IgG Antibody, Serum
44060 St. Louis Encephalitis IgG Antibody, Serum
28237 West Nile Virus IgG Antibody, Serum - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL
Pediatric Collection: 0.15 mL - Fasting:
No - Spec Collect:
Routine venipuncture
Parallel testing is preferred
Label samples as: "acute" or "convalescent"
Convalescent samples must be received within 30 days from receipt of acute samples - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube - Spec Store Transport:
Refrigerated - Spec Stability:
After separation from cells:
48 hours room temperature (20-30°C)
2 weeks refrigerated (2-8°C)
1 year frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Severely lipemic, hemolyzed, or contaminated sample - Methodology:
Indirect Fluorescent Antibody (IFA) - Clinical Significance:
Refer to:
- Reference Range:
- Western Equine Encephalitis Antibody, IgG by IFA, Serum
- Less than 1:16
- Interpretive Data:
- A positive result for IgG may indicate current or past infection. Eastern equine encephalitis and western equine encephalitis viruses are both related and will show cross-reactivity. Initial infection by one of these viruses will show a specific rise in titer which is higher than titers against any other viruses of the group. A subsequent infection by another virus within this group, however, will boost the titer against the initial infecting virus (anamnestic response) and make a specific diagnosis difficult.
- Note:
- Serum specimens drawn within the first two weeks after onset are variably negative for IgG antibody and should not be used to exclude the diagnosis of arboviral disease. Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change (fourfold difference in titer) on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.
- Day Run:
Tue, Fri - Time Reported:
2-6 days - Test Type:
INFECTIOUS ANTIBODY