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Test Name:
C1Q Binding Assay
- SBMF No:
44042 - Performance Lab Name:
ARUP Laboratories - Test Mnemonic:
C1Q- - ABN:
Not required - CPT Code:
86332 - LOINC Code:
27831-7 - Ref Lab Test No:
0050301 - Also Known As:
Circulating Immune Complex Detection by C1q Binding
Immune Complex Assay (C1Q) - Also See:
45557 Immune Complex Panel
44531 Raji Cell Immune Complex Assay - Spec Type:
Serum - Spec Container:
Red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL
Pediatric Collection: 0.15 mL - Fasting:
No - Spec Collect:
Do not use gel-barrier tubes for specimen collection
Routine venipuncture
Separate samples must be submitted when multiple tests are ordered - Spec Process:
Let stand on clot for 2 hours
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube and freeze - Spec Store Transport:
Frozen - Spec Stability:
After separation from cells:
2 hours room temperature (20-30°C)
Refrigerated (2-8°C): Unacceptable
2 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Non-frozen sample - Spec Remarks:
If ordered in conjunction with a Raji Cell Assay, draw two plain red tubes and submit two 1.0 mL frozen serum samples for testing
CRITICAL FROZEN – Separate samples must be submitted when multiple tests are ordered - Methodology:
Enzyme-Linked Immunosorbent Assay (ELISA) - Clinical Significance:
Refer to:
- Reference Range:
- C1Q Binding Assay
- Less than 4 µgE/mL is considered negative for circulating complement binding immune complexes
- Interpretive Data:
- Circulating immune complexes may be found without any evident pathology and positive results do not necessarily implicate the immune complex in a disease process.
- Day Run:
Mon, Thu - Time Reported:
2-9 days - Test Type:
IMMUNOLOGY
