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Test Name:
Interleukin 6, by ELISA


  • SBMF No:
    43208
  • Performance Lab Name:
    Quest Diagnostics Nichols Institute
  • Test Mnemonic:
    IL6 ELISA
  • ABN:
    Required – Not FDA-Approved
  • CPT Code:
    83520
  • LOINC Code:
    19146-0
  • Ref Lab Test No:
    34473X
  • Also Known As:
    IL-6
    IL6
    Interleukin-6 by Highly Sensitive ELISA
  • Also See:
    44062 Interleukin 6, by MAFD
  • Spec Type:
    Plasma
  • Spec Container:
    Lavender top (EDTA) tube
  • Alt Spec Type:
    Serum
  • Alt Spec Container:
    Gold top (SST) or red top (serum) tube
  • Pref Vol:
    1.0 mL
  • Min Vol:
    0.5 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube and freeze
  • Spec Store Transport:
    Frozen
  • Spec Stability:
    After separation from cells:
    24 hours room temperature (20-30°C)
    48 hours refrigerated (2-8°C)
    7 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Thawed sample
    Sample received room temperature or refrigerated
  • Spec Remarks:
    Cytokine levels may demonstrate diurnal variation. Recommend cytokine levels be determined at the same time of day for improved longitudinal comparison.
  • Methodology:
    Enzyme-Linked Immunosorbent Assay (ELISA)
  • Use:
    To evaluate the level of IL-6 in serum or EDTA plasma samples
  • Reference Range:
    Interleukin-6, Highly Sensitive ELISA
    0.31-5.00 pg/mL
  • Additional Test Info:
    This test is performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute, Valencia. This test should not be used for diagnosis without confirmation by other medically established means.
  • Day Run:
    Varies; 1 run per week
  • Time Reported:
    Reports in 3 days
  • Test Type:
    IMMUNOLOGY