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Test Name:
Skin Total Autoantibodies, Inter-Epithelial and Dermal-Epidermal, by IFA


  • SBMF No:
    43101
  • Performance Lab Name:
    Quest Diagnostics Nichols Institute
  • Test Mnemonic:
    SKIN ABS
  • ABN:
    Not required
  • CPT Code:
    86256x2
  • Ref Lab Test No:
    1114
  • Test Includes:
    Skin Autoantibodies, Intercellular
    Skin Autoantibodies, Dermal-Epidermal
  • Also Known As:
    Epidermal Antibodies
    Pemphigus/Pemphigoid, Skin Antibody
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Alt Spec Type:
    Plasma
  • Alt Spec Container:
    Lavender top (EDTA) tube, pale yellow top (ACD ) tube, or green top (heparin) tube
  • Pref Vol:
    1.0 mL
  • Min Vol:
    0.4 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    2 days room temperature (20-30°C)
    1 week refrigerated (2-8°C)
    2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Methodology:
    Indirect Fluorescent Antibody (IFA)
  • Use:
    Skin antibodies (inter-epithelial) are found in 90% of patients with Pemphigus vulgaris and P. foliaceus. Dermal-epidermal skin antibodies are antibodies found in 90% of patients with Bullous pemphigoid and 90% of Cicatricial pemphigoid.
  • Reference Range:

    Skin Autoantibodies, Intercellular:
    < 1:10 titer

    Skin Autoantibodies, Dermal-Epidermal:
    < 1:10 titer

  • Day Run:
    Wed
  • Time Reported:
    Same day
    This is a sendout test. Please note that turnaround time is defined as the anticipated time from set up day to results release.
  • Test Type:
    IMMUNOLOGY

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