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Test Name:
Pepsin Assay, Airway Fluid
- SBMF No:
42280 - Performance Lab Name:
Nemours Biomedical Research, GI Clinical Lab, Alfred I. duPont Hospital for Children - Test Mnemonic:
FAIRP - ABN:
Not required - CPT Code:
82657 - Also Known As:
Airway Pepsin Assay
Gastric Pepsin Assay - Spec Type:
Airway fluid (tracheal or bronchial secretion) - Spec Container:
Plastic transport tube with threaded cap - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Spec Collect:
Collected by physician or nursing personnel
To collect tracheal secretion: Attach a LUKI trap to a suction catheter, pass the catheter down the endotracheal tube, and apply suction to the catheter as the catheter is withdrawn. If the secretion is too thick to be withdrawn, instill saline (2 cc) to the tracheal tube prior to aspirating the secretion.
To collect bronchial secretion: Bronchial secretion can be collected using a standard collection protocol. Collect 1-2 cc bronchial washing fluid in a plain specimen tube.
Avoid over-diluting the secretion with a large amount of saline during the collection.
- Spec Process:
Transfer fluid to plastic transport tube and freeze immediately after collection - Spec Store Transport:
Frozen
Ship on dry ice - Methodology:
ELISA with Immunofluoresence - Use:
Assess pulmonary aspiration - Clinical Significance:
Gastric pepsin detected in the airway of patients is a specific and sensitive marker to assess pulmonary aspiration. The GI Clinical Lab at Nemours offers a sensitive, reliable enzymatic test to detect gastric pepsin in the airway samples. The assay has high specificity as it detects pepsin A that is exclusively expressed in the gastric mucosa. - Reference Range:
By report - Additional Test Info:
This test is referred through Mayo Medical Laboratories - Day Run:
Mon-Fri - Time Reported:
Within 7 days - Test Type:
ENZYME
