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Test Name:
HEPTIMAX™ Hepatitis C Virus RNA


  • SBMF No:
    41576
  • Performance Lab Name:
    Quest Diagnostics Nichols Institute
  • Test Mnemonic:
    HEPTIMAX
  • ABN:
    Not required
  • CPT Code:
    87522
  • LOINC Code:
    19146-0
  • Ref Lab Test No:
    10565X
  • Also Known As:
    Hepatitis C Virus RNA HEPTIMAX™
    HEPTIMAX™ HCV RNA
  • Also See:
    25106 Hepatitis C Virus RNA, Quantitative, by RT-PCR
  • Spec Type:
    Plasma
  • Spec Container:
    Pearl white top (PPT) (EDTA + gel barrier material), lavender top (EDTA), or pale yellow top (ACD-B) tube
  • Alt Spec Type:
    Serum
  • Alt Spec Container:
    Gold top (SST) or red top (serum) tube
  • Pref Vol:
    3.0 mL
  • Min Vol:
    1.6 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    IMPORTANT: Sample must be removed from cells within 6 hours of collection time
    It is optimal to process samples immediately after collection; however, samples must be processed at least within 6 hours of collection time or they will be rejected
    Separate plasma from whole blood by centrifugation at 800 to 1600 xg for 20 minutes at room temperature
    Transfer plasma to a properly identified, sterile, polypropylene, screw-capped vial
    Freeze and transport frozen
  • Spec Store Transport:
    Frozen
  • Spec Stability:
    Room temperature (20-30°C): Unacceptable
    48 hours refrigerated (2-8°C)
    42 days frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Heparinized sample
    Sample submitted in original collection tube
  • Methodology:
    Polymerase Chain Reaction (PCR)
    Transcription-Mediated Amplification (TMA)
  • Use:
    Test is used for the Quantitative detection of Hepatitis C virus (HCV) RNA. This test combines PCR and TMA for the detection of the broadest range of HCV (5-69,000,000 IU/mL).
  • Reference Range:

    HEPTIMAX™ HCV RNA
    < 5 IU/mL
    < 0.7 LogIU/mL

    Note:
    In response to client requests, results > or = 1,000,000 are reported in a modified exponential notation. For example, a result of 1,000,000 would be reported as 1.00E6, and a result of 42,000,000 would be reported as 42.0E6.
    Reportable Ranges:
    5-69,000,000 IU/mL
    0.70-7.84 Log IU/mL
    The method used in this test is Real-Time RT-PCR of the 5' UTR of the HCV genome.
    This test is performed using COBAS® Ampliprep/COBAS® Taqman® HCV Test Kit (Roche Molecular Systems, Inc.)

  • Day Run:
    Mon-Fri
  • Time Reported:
    4 days
  • Test Type:
    INFECTIOUS ORGANISM

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