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Test Name:
Rickettsia rickettsii DNA, by RT-PCR
- SBMF No:
33077 - Performance Lab Name:
Focus Diagnostics - Test Mnemonic:
RMSF PCR - ABN:
Not required - CPT Code:
87798 - LOINC Code:
19146-0 - Ref Lab Test No:
45500 (Quest# S51477) - Also Known As:
Rocky Mountain Spotted Fever - Spec Type:
Whole blood - Spec Container:
Lavender top (EDTA) or pale yellow top (ACD-A or B) tube - Pref Vol:
1.0 mL - Min Vol:
0.7 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Gently invert tube to mix blood with the anticoagulant
Do not centrifuge
Do not remove plasma from cells
Forward entire tube of unprocessed whole blood to the laboratory - Spec Store Transport:
Refrigerated - Spec Stability:
48 hours room temperature (20-30°C)
7 days refrigerated (2-8°C)
Frozen (-20°C) Unacceptable - Methodology:
Real-Time Polymerase Chain Reaction (RT-PCR) - Clinical Significance:
According to the CDC, Rocky Mountain spotted fever is the most severe and most frequently reported rickettsial illness in the United States. Infection is transmitted by ixodid (hard) ticks carried by dogs and other mammal species. Initial signs and symptoms of the disease include sudden onset of fever, headache, and muscle pain, followed by development of rash. The disease can be fatal without prompt treatment. - Reference Range:
Not detected - Additional Test Info:
This test is referred through Quest Diagnostics Nichols Institute. - Day Run:
Varies - Time Reported:
Varies - Test Type:
INFECTIOUS ORGANISM
