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Test Name:
Estradiol, Free, Serum
- SBMF No:
33054 - Performance Lab Name:
Quest Diagnostics Nichols Institute - Test Mnemonic:
E2 FREE - ABN:
Not required - CPT Code:
82670x2 - LOINC Code:
19146-0 - Ref Lab Test No:
36169 - Test Includes:
Estradiol, Free
Estradiol, % Free
Estradiol - Spec Type:
Serum - Spec Container:
Red top (serum) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
Do not use gel-barrier tubes for specimen collection
Routine venipuncture - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube and freeze - Spec Store Transport:
Frozen - Spec Stability:
After separation from cells:
4 hours room temperature (20-30°C)
3 days refrigerated (2-8°C)
2 years frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Grossly hemolyzed specimen - Methodology:
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Equilibrium Dialysis - Clinical Significance:
Much of Estradiol is bound to proteins. The unbound portion and Estradiol bound to proteins with low affinity reflect the Free concentration. The Free Estradiol may better correlate with medical conditions than the Total Estradiol concentrations. - Reference Range:
Estradiol, Free
Females
Follicular Stage: 0.43-5.03 pg/mL
Mid-cycle Stage: 0.72-5.89 pg/mL
Luteal Stage: 0.40-5.55 pg/mL
Postmenopausal: < or = 0.38 pg/mL
Males (Adult): < or = 0.45 pg/mL
Estradiol
Females
Follicular Stage: 39-375 pg/mL
Mid-cycle Stage: 94-762 pg/mL
Luteal Stage: 48-440 pg/mL
Postmenopausal: < or = 10 pg/mL
Males (Adult): < or = 29 pg/mL - Day Run:
Sets up 4 days a week - Time Reported:
Reports in 4 days
This is a sendout test. Please note that turnaround time is defined as the anticipated time from set up day to results release. - Test Type:
HORMONE
