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Test Name:
Estrogens, Fractionated, Serum or Plasma
- SBMF No:
30127 - Performance Lab Name:
Manual Lab - Test Mnemonic:
ESTROGENS - ABN:
Not required - CPT Code:
82671 - LOINC Code:
2243-4; 2258-2; 2254-1 - Test Includes:
Estradiol
Estrone
Estrogens, Total (Calculation) - Also See:
30085 Estradiol, Serum or Plasma
30094 Estrone, Serum or Plasma - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Lavender top (EDTA) tube - Pref Vol:
1.5 mL - Min Vol:
0.8 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube - Spec Store Transport:
Refrigerated (24 hours) or frozen - Spec Stability:
24 hours refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Hemolyzed or lipemic sample - Spec Remarks:
Separate serum or plasma from cells ASAP or within 2 hours of collection - Methodology:
Radioimmunoassay (RIA)
Electrochemiluminescent Immunometric Assay (ECLIA) - Use:
Evaluate ovarian estrogen producing tumor in the premenarcheal and postmenopausal patients.
Evaluate estrogen excess in males - Clinical Significance:
Estrone (E1) and estradiol (E2) are two major naturally occurring estrogens in pre-menopausal, non-pregnant women. Estradiol is the most biologically active estrogen. Approximately 95% of estradiol is produced in the ovary (follicle, corpus luteum). Estrone is produced primarily from androstenedione originating from either the adrenal cortex or gonads. In premenopausal adult women, more than 50% of estrone is secreted by the ovary. In prepubertal children, men and postmenopausal women, estrone is mainly derived from peripheral tissue conversion of androstenedione. Interconversion of estrone and estradiol also occurs in peripheral tissues. During pregnancy, large amounts of estrone and estradiol are synthesized in the placenta.
Estrone is of little significance in the ovulating women. However, it becomes the predominant estrogen in the absence of functioning follicles in the postmenopausal years. Although the physiologic role of endogenous estrone is not well defined, it is a primary estrogenic component of several pharmaceutical preparations for estrogen replacement therapy. Estrone can serve as an important clinical marker of estrogenicity in postmenopausal women on estrogen replacement therapy.
The former method for quantitating total estrogens used a non-specific RIA method that measured estradiol and estrone, but also measured their metabolites. Consequently, total estrogen values by such a method reflected not just ovarian production of these estrogens, but also hepatic metabolism of the estrogens. The new test will use a specific assay for estrone and a separate specific assay for estradiol. Using this approach, the result will better reflect the status of estrogen production in vivo. - Reference Range:
––– Fractionated Estrogens ––– Age Group Estrone (E1) Estradiol (E2) Total Estrogens (calc) Prepubertal 0-24 pg/mL 0-36 pg/mL 0-50 pg/mL Male 0-64 pg/mL 0-44 pg/mL 0-108 pg/mL Female, Post Menopausal 14-103 pg/mL 0-44 pg/mL 14-147 pg/mL Female, Follicular Phase 37-138 pg/mL 0-177 pg/mL 37-315 pg/mL Female, Mid-Cycle 60-229 pg/mL 48-388 pg/mL 108-617 pg/mL Female, Luteal Phase 50-114 pg/mL 31-247 pg/mL 81-361 pg/mL - Day Run:
Mon, Thu - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
HORMONE
