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Test Name:
Estradiol, Serum or Plasma
- SBMF No:
30085 - Performance Lab Name:
Automated Lab - Test Mnemonic:
ESTRAD - ABN:
Not required - CPT Code:
82670 - LOINC Code:
2243-4 - Also Known As:
E2, Unconjugated - Also See:
30127 – Estrogens, Fractionated - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Lavender top (EDTA), Green top (heparin), or Gray top (fluoride/oxalate) tube - Pref Vol:
1.0 mL - Min Vol:
0.3 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated - Spec Stability:
1 week refrigerated (2-8°C)
6 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Heat-inactivated sample - Methodology:
Electrochemiluminescent Immunoassay (ECLIA) - Use:
Assess hypothalamic and pituitary function; assess ovarian failure and follicular maturation
Amenorrhea work-up: low result, if gonadotropins high, may indicated ovarian failure; if gonadotropins and estradiol low, consider hypothalamic or pituitary disorder. In males, E2 measurements may be helpful in the assessment of unexplained gynecomastia
In females, E2 measurements are frequently utilized to document hypoestrogenism in cases of cases of delayed puberty, primary and secondary amenorrhea, and menopause
Gonadotropin measurements must be utilized to localize the cause of hypoestrogenism to the ovaries or to a hypothalamic-pituitary source
E2 concentrations greater than 1000 pg/mL are usually observed only during pregnancy or in rare cases of estrogen secreting tumors - Clinical Significance:
Estrogens are responsible for the development of the secondary female sex characteristics. Together with gestagens, they control all the important female reproductive processes.The biologically most active estrogen is 17b-estradiol. This is a steroid hormone having a molecular weight of 272 daltons. Estrogens are produced primarily in the ovary (follicle, corpus luteum), but small quantities are also formed in the testes and in the adrenal cortex. During pregnancy, estrogens are mainly formed in the placenta. About 98% of estradiol is bound to transport proteins (SHBG = sex hormone binding globulin).Estrogen secretion is biphasic during the menstrual cycle. The determination of estradiol is utilized clinically in the elucidation of fertility disorders in the hypothalamus-pituitary-gonad axis, gynecomastia, estrogen-producing ovarian and testicular tumors and in hyperplasia of the adrenal cortex. Further clinical indications are the monitoring of fertility therapy and determining the time of ovulation within the framework of in vitro fertilization (IVF). - Reference Range:
Children:(females 0-12 yrs) Up to 36 pg/mL
Males:(all ages) Less than 45 pg/mL
Females, Postmenopausal: Less than 46 pg/mL
Females, Follicular: Less than 178 pg/mL
Females, Mid Cycle: 48-388 pg/mL
Females, Luteal: 31-247 pg/mL - Additional Test Info:
In patients receiving therapy with high biotin doses (i.e., greater than 5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration.The risk of interference from potential immunological interactions between test components and rare sera has been minimized by the inclusion of suitable additives. In rare cases interference due to extremely high titers of antibodies to streptavidin can occur.For diagnostic purposes, Estradiol II findings should always be assessed in conjunction with the patient’s medical history, clinical examination and other findings. - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
HORMONE
