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Test Name:
Growth Hormone Suppression Panel
- SBMF No:
30070 - Performance Lab Name:
Manual Lab - Test Mnemonic:
GH SUPP - ABN:
Not required - CPT Code:
80430 - LOINC Code:
12253-1; 12646-6; 12610-2; 1558-6; 14146-5; 14144-0; 56500-2 - Test Includes:
GH, Baseline
GH, 60 Minutes
GH, 120 Minutes
GH, 180 Minutes
Glucose, Fasting
Glucose, 60 Minutes
Glucose, 120 Minutes - Also See:
30029 Growth Hormone
30069 Growth Hormone Stimulation Panel - Spec Type:
Serum –plus– Plasma - Spec Container:
Two (2) tubes: Gold top (SST) or red top (serum) tube –plus– light green top (PST) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Fasting:
Yes - Patient Prep:
Fasting at least 12 hours
Patient should be at rest 30 minutes prior to collection - Spec Collect:
Routine venipuncture
Collect at specified times:
– Baseline/Fasting (pre-glucose dose): Serum - plus - plasma
– 60 Minute (1 Hour) post-glucose dose: Serum - plus - plasma
– 120 Minute (2 Hour) post-glucose dose: Serum - plus - plasma
– 180 Minute (3 Hour) post-glucose dose: Serum only - Spec Process:
Serum tubes:
– Allow specimen to clot thoroughly prior to centrifugation
– Promptly centrifuge 15 minutes
– Immediately transfer serum to separate plastic tube and freeze
Plasma tubes: Promptly centrifuge 15 minutes and refrigerate - Spec Store Transport:
Frozen (serum GH samples)
Refrigerated (plasma glucose samples) - Spec Stability:
Growth Hormone samples:
8 hours refrigerated (2-8°C)
2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
Glucose samples:
1 week refrigerated (2-8°C) - Spec Reject:
Plasma or serum in contact with RBC more than 1 hour
Severely hemolyzed or severely lipemic sample - Methodology:
Chemiluminescent Immunometric Assay
Photometric - Use:
Pituitary Hormone Secretion testing - Clinical Significance:
Human growth hormone (HGH; somatotropin) is a 22,000 Dalton (22k) molecular weight polypeptide hormone secreted by the anterior pituitary. An HGH variant of 20,000 Dalton molecular weight is also present in serum. The 22k HGH is the predominant part of the total HGH in serum.
Growth hormone-releasing hormone specifically stimulates the synthesis and release of HGH by somatotropic cells of the anterior pituitary gland. Human growth hormone stimulates the liver to produce somatomedins, a group of hormones that directly promote growth. HGH indirectly affects many metabolic processes via the somatomedins including cellular uptake of amino acids and release of fatty acids from tissue stores.
There are many stimulatory and inhibitory factors for secretion of HGH. Inhibitory factors include glucose, cortisol, free fatty acids and somatostatin. Stimulatory factors include exercise, hypoglycemia, arginine, L-dopa, glucagon, histamine, vasopressin, catacholamine, slow wave sleep and stress. The measurement of serum HGH has been used in diagnosing hyposomatotropism and hypersomatotropism. The clinical disorders of hyposomatotropism include dwarfism and unattained growth potential. The clinical disorders of hypersomatotropism include gigantism in children and acromegaly in adults. Acromegaly is usually caused by pituitary adenomas and rarely caused by pancreatic tumors.
A single measurement of HGH is usually of limited clinical value because HGH secretion is pulsatile. To assess the clinical status of HIGH disorders, provocative or suppressive stimuli have been used. Provocative stimuli for an increase in serum HGH levels include exercise, sleep, insulin, clonidine, arginine, L-dopa and glucagon. For suppression of serum HGH levels, a standard glucose tolerance test is usually used. If hyposomatotropism is suspected a provocative test is used. If hypersomatotropism is suspected a glucose tolerance test is used. Blood samples can be taken before the stimulus and 0, 30, 60, 90 and 120 minutes after the stimulus.
- Reference Range:
In response to a glucose suppression test, a response of falling to < 2 ng/mL is shown in normal individuals.
Patients with acromegaly fail to show this suppression and at times show a paradoxical increase in GH levels. - Day Run:
Thu - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
HORMONE
