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Test Name:
Vitamin D, 25-Hydroxy, Total
- SBMF No:
30054 - Performance Lab Name:
Manual Lab - Test Mnemonic:
VIT D25- - ABN:
May be required – Medical Necessity - CPT Code:
82306 - LOINC Code:
62292-8 - Also Known As:
25-OH D
25-Hydroxyvitamin D - Also See:
31404 Vitamin D2 and D3, 25-Hydroxy, by LC-MS/MS - Spec Type:
Serum - Spec Container:
Gold top (SST) or Red top (serum) tube - Pref Vol:
0.5 mL - Min Vol:
0.3 mL - Fasting:
Recommended, but not required - Spec Collect:
Routine venipuncture - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated - Spec Stability:
5 days refrigerated (2-8°C)
9 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Severely hemolyzed or severely lipemic specimen
Specimens other than serum - Methodology:
Chemiluminescent Immunoassay (CLIA) - Use:
Assess status of vitamin D
Rule out vitamin D deficiency as a cause of bone diseases
25-hydroxy vitamin D intoxication - Clinical Significance:
The determination of 25-OH vitamin D in serum or plasma is used in the assessment of vitamin D sufficiency. Since 25-OH vitamin D is the predominant circulating form of vitamin D in the normal population, it is considered to be the most reliable index of vitamin D status. The measurement of 25-OH vitamin D is becoming increasingly important in the management of patients with various disorders of calcium metabolism associated with rickets, neonatal hypocalcemia, pregnancy, nutritional and renal osteodystrophy, hypoparathyroidism, and postmenopausal osteoporosis. - Reference Range:
25-OH vitamin D levels for bone and overall health in healthy individuals: Severe deficiency <10 ng/mL Inadequate 10-29 ng/mL Sufficient levels 30-80 ng/mL Excess >80 ng/mL Toxic Consistently >150 ng/mL - Additional Test Info:
- Day Run:
Mon-Fri (daily) - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
CHEMISTRY
