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Test Name:
Vitamin B12 with Serum Folate


  • SBMF No:
    30040
  • Performance Lab Name:
    Automated Lab
  • Test Mnemonic:
    B12/FOL
  • ABN:
    Not required
  • CPT Code:
    82607; 82746
  • LOINC Code:
    2132-9; 2284-8
  • Test Includes:
    Vitamin B12, Serum
    Folate, Serum
  • Also Known As:
    B12 with Serum Folate
    Vitamin B12 with Serum Folate
  • Also See:
    30041 – Vitamin B12
    30037 – Folate, Serum
    30039 – Vitamin B12 with RBC Folate
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Pref Vol:
    2.0 mL
  • Min Vol:
    0.6 mL
  • Fasting:
    Recommended
  • Patient Prep:
    Fasting 12 hours preferred
    Testing not recommended within 1 month of B12 injection or 8 hours of last biotin dose
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum to light-protected (amber) plastic tube
  • Spec Store Transport:
    Refrigerated and protected from light
  • Spec Stability:
    Room temperature (20-30°C): Unacceptable
    1 week refrigerated (2-8°C)
    2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Hemolyzed sample
  • Spec Remarks:
    Preferred sample is serum, protected from light and refrigerated (2-8°C)
  • Methodology:
    Electrochemiluminescent Immunoassay (ECLIA)
  • Use:
    See individual test listings
  • Clinical Significance:
    See individual test listings
  • Reference Range:
    Folate, Serum:
        – Normal: Equal to or greater than 4.2 ng/mL
        – Borderline Deficient: 2.1-4.1 ng/mL
        – Deficient: Less than 2.1 ng/mL
    Vitamin B12: 215-1095 pg/mL
  • Day Run:
    Sun-Sat (daily)
  • Time Run:
    As received
  • Time Reported:
    4 hours
  • Test Type:
    HEMATOLOGY