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Test Name:
Vitamin B12 with RBC Folate
- SBMF No:
30039 - Performance Lab Name:
Automated Lab - Test Mnemonic:
B12/RBC - ABN:
Not required - CPT Code:
82607; 82747 - Test Includes:
Vitamin B12, Serum
Folate, Whole Blood - Also Known As:
B12 with RBC Folate
Folate, RBC with Vitamin B12 - Also See:
30041 – Vitamin B12
30036 – Folate, RBC
30040 – Vitamin B12 with Serum Folate - Spec Type:
Serum - plus - whole blood (EDTA) - Spec Container:
2 tubes: Gold top (SST) or red top (serum) tube - plus - lavender top (EDTA) tube - Alt Spec Type:
Serum - plus - whole blood (EDTA) - Alt Spec Container:
2 tubes: Red top (serum) tube - plus - lavender top (EDTA) tube - Pref Vol:
1.0 mL serum - plus - 2.0 mL EDTA whole blood - Min Vol:
0.5 mL serum - plus - 0.5 mL EDTA whole blood - Fasting:
Recommended - Patient Prep:
Fasting 12 hours preferred
Testing not recommended within 1 month of B12 injection or 8 hours of last biotin dose - Spec Collect:
Routine venipuncture
Immediately after collection, gently invert lavender top tube to mix well
Patient's hematocrit value (required for folate testing):
– Perform hematocrit on lavender tube, or
– If patient has not been transfused, use hematocrit result obtained within past 3 days, or
– Send separate lavender top tube to SBMF for hematocrit testing - Spec Process:
Serum sample
– Clot 30 minutes
– Promptly centrifuge 15 minutes
Whole blood sample
– Do not centrifuge
– Record patient’s hematocrit result on tube label and on test requisition
– Refrigerate (2-8°C) entire tube of EDTA whole blood and transport refrigerated - Spec Store Transport:
Refrigerated and protected from light - Spec Stability:
Room temperature (20-30°C): Unacceptable
1 week refrigerated (2-8°C)
2 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Methodology:
See individual test listings - Use:
See individual test listings - Clinical Significance:
See individual test listings - Reference Range:
Vitamin B12: 215-1095 pg/mL
RBC folate: 160-600 ng/mL - Day Run:
Mon-Fri - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
HEMATOLOGY
