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Test Name:
Catecholamines, Fractionated, Urine, 24-Hour


  • SBMF No:
    29130
  • Performance Lab Name:
    Manual Lab
  • Test Mnemonic:
    U CAT
  • ABN:
    Not required
  • CPT Code:
    82384
  • LOINC Code:
    2218-6; 13362-9; 3167-4; 20624-3; 2162-6; 19150-2; 32015-0; 2232-7; 2667-4; 2668-2; 2217-8
  • Test Includes:
    Epinephrine
    Norepinephrine
    Dopamine
  • Also Known As:
    Epinephrine, Urine
    Fractionation, Urinary Free Catecholamines
    Free Catecholamine Fractionation
    Noradrenaline
    Norepinephrine, Urine
    Dopamine, Urine
    Adrenaline
  • Spec Type:
    Urine, 24-hour
  • Spec Container:
    Plastic container with tightly fitting lid
  • Pref Vol:
    10.0 mL aliquot from well-mixed 24-hour collection
        Note: Send urine aliquot with notation of 24-hour volume on aliquot label and on requisition
  • Min Vol:
    4.0 mL aliquot from well-mixed collection
  • Fasting:
    No
  • Spec Collect:
    To begin collection of timed specimen, have patient urinate, noting date and exact time
    Discard this first portion of urine, but document date and time of voiding on container label and test requisition as "start" of timed specimen
    Save all urine voided during designated time period, including portion voided at exact end of time period
    IMPORTANT: Refrigerate entire specimen (2-8°C) during and after collection to prevent bacterial growth
    Document date and time of final voiding on label and requisition as "end" of timed specimen
  • Spec Process:
    Upon completion of collection, mix entire specimen well and note 24-hour volume
    Acidify urine sample with 6N Hydrochloric Acid to pH between 1.0 and 3.0 when received in lab
    Pour 10.0 mL of urine into separate plastic container
    Refrigerate urine aliquot and transport refrigerated
    Send urine aliquot with notation of 24-hour urine volume on aliquot label and on requisition
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Methodology:
    High Performance Liquid Chromatography (HPLC)
  • Use:
    Elevations in urinary catecholamines may be an indication of a pheochromocytoma or neuroblastoma, progressive muscular dystrophy, or myasthenia gravis. A decrease may be observed in familial dysautomia, malnutrition, or transection of cervical spinal cord
  • Clinical Significance:
    Pheochromocytoma, a tumor of the chromaffin tissue, is associated with the presence of great increased catecholamine concentrations. Elevated catecholamines have also been found in patients with other tumors of neural tube origin, such as neuroblastomas and ganglioneuroblastomas. Degradation products of catecholamines, normetanephrine, metanephrine, and vanilmandelic acid (VMA) may be measured in conjunction with the catecholamines. These compounds are present in the urine in much larger quantities than epinephrine, norepinephrine, or dopamine.
  • Reference Range:

    24 Hour Catecholamines, Urinary, Free
    Epinephrine:         Up to 1 year
    1-2 years
    2-4 years
    4-10 years
    10-16 years
    16 years +    		     0-2.5 μg/24 hours
    0-3.5 μg/24 hours
    0-6.0 μg/24 hours
    0.2-10 μg/24 hours
    0.5-20 μg/24 hours
    0-22 μg/24 hours
    Norepinephrine:     Up to 1 year
    1-2 years
    2-4 years
    4-7 years
    7-10 years
    10 years +    		     0-10 μg/24 hours
    1-17 μg/24 hours
    4-29 μg/24 hours
    8-45 μg/24 hours
    13-65 μg/24 hours
    12-85 μg/24 hours
    Dopamine:            Up to 1 year
    1-2 years
    2-4 years
    4 years +    		     0-85 μg/24 hours
    10-140 μg/24 hours
    40-260 μg/24 hours
    65-400 μg/24 hours
  • Additional Test Info:
    Record total specimen volume, collection times, and pH [if required] on test requisition
  • Day Run:
    Tue, Thu, Sat
  • Time Run:
    7:00 am
  • Time Reported:
    5:00 pm, next day
  • Test Type:
    HORMONE

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