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Test Name:
Glucose Tolerance with Insulin Levels (Fasting and 2-Hour Post-Glucose [75 g] dose)
- SBMF No:
29073 - Performance Lab Name:
Automated Lab - Test Mnemonic:
GTT2+INSUL - ABN:
May be required – Medical Necessity - CPT Code:
82947; 82950; 83525x2 - LOINC Code:
27873-9; 12610-2; 47659-8; 1558-6 - Test Includes:
Glucose, fasting
Insulin, fasting
Glucose, 2-hour post-glucose dose
Insulin, 2-hour post-glucose dose - Spec Type:
Plasma - Spec Container:
Light green top (PST) tube - Alt Spec Type:
Serum - Alt Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
3.0 mL - Min Vol:
1.0 mL - Fasting:
Yes - Patient Prep:
Fasting at least 12 hours; fasting should not exceed 14 hours - Spec Collect:
Routine venipuncture
Collect pre-glucose dose samples:
- Fasting sample for glucose and insulin
Administer 75 g glucose dose to patient
Collect 2-hour post-glucose dose samples
- 2--hour sample for glucose and insulin - Spec Process:
PST tubes:
- Promptly centrifuge 15 minutes
- Transfer plasma to a separate plastic tube and freeze
Gold top or red top tubes:
- Clot 30 minutes
- Promptly centrifuge 15 minutes
- Within 60 minutes of collection, transfer serum to separate plastic tube and freeze - Spec Store Transport:
Frozen - Spec Stability:
1 week frozen (-20°C) – do not use frost-free units that undergo repeated freeze/thaw cycles - Methodology:
Glucose: Enzymatic
Insulin: Electrochemiluminescent Immunoassay (ECLIA) - Test Type:
CHEMISTRY
