South Bend Medical Foundation - Your Community Laboratory

test directorySearch Results

Click here to search again.

Test Name:
Gliadin IgA and IgG Antibodies

• SBMF No:
  28193
• Performance Lab Name:
  Immunology
• Test Mnemonic:
  GLIAD ABS
• ABN:
  Not required
• CPT Code:
  83516x2
• LOINC Code:
  20495-8; 5170-6
• Test Includes:
  Gliadin IgA Antibody
  Gliadin IgG Antibody
• Also See:
  35198 Celiac Serology Profile
• Spec Type:
  Serum
• Spec Container:
  Gold top (SST) or red top (serum) tube
• Pref Vol:
  1.0 mL
• Min Vol:
  0.5 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  Clot 30 minutes
  Promptly centrifuge 15 minutes
  Immediately transfer serum to separate plastic tube
  Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  7 days refrigerated (2-8°C)
  Greater than 7 days, freeze (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
• Spec Reject:
  Severely lipemic, contaminated, or hemolyzed sample
• Methodology:
  Enzyme-Linked Immunosorbent Assay (ELISA)
• Use:
  Aid in diagnosis of certain gluten sensitive enteropathies such as celiac disease and dermatitis herpetiformis
• Clinical Significance:
  The gliadin antibodies (GA) are IgG and IgA antibodies against a group of proteins found in the gluten of wheat and rye grains. The ELISA test for GA is a reliable screening tool for the evaluation of asymptomatic celiac disease in pre- pubertal children with short stature. Celiac disease results from an intolerance to dietary gluten, resulting in small intestinal villous atrophy with subsequent malabsorption and malnutrition. Classic symptoms include: diarrhea, weight loss, abdominal pain and distention, fatigue, oral ulceration, bleeding tendency, proximal myopathy, and bone and joint pain. Short stature, delayed puberty, arthritis, or arthralgia may also result from the untreated disease. GA are useful in screening populations at risk for celiac disease and other gluten- sensitive enteropathies such as dermatitis herpetiformis, and in monitoring patient compliance to a gluten-free diet. In celiac disease, IgG antibodies are more sensitive than IgA antibodies, but IgA antibodies are more specific than IgG antibodies. The level of IgA antibodies decreases with a gluten-free diet. IgA and IgG antibodies rise significantly during gluten challenge, sometimes several months before clinical relapse.
• Reference Range:
  0.0-7.0 U/mL

  Interpretive Data:

  < 7.0 7.0 - 10.0 > 10.0

  U/mL = Negative U/mL = Equivocal U/mL = Positive

  Testing is being performed on the Phadia Immunocap 250

• Day Run:
  Wed, Sat
• Time Run:
  8:00 am
• Time Reported:
  5:00 pm
• Test Type:
  IMMUNOLOGY