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Test Name:
Heparin-Associated Antibody, PF4 ELISA


  • SBMF No:
    28144
  • Performance Lab Name:
    Immunology
  • Test Mnemonic:
    HEP PF4
  • ABN:
    Not required
  • CPT Code:
    86022
  • LOINC Code:
    6002-0
  • Also Known As:
    PF4 Antibody
    Heparin Induced Thrombocytopenia (HIT)
    HIT
  • Also See:
    28264 Heparin-Associated Antibody, PF4 ELISA, with Serotonin Release Assay – Enoxaparin (LMWH) if Indicated
    28265 Heparin-Associated Antibody, PF4 ELISA, with Serotonin Release Assay – Unfractionated Heparin if Indicated
  • Spec Type:
    Serum
  • Spec Container:
    Red top (serum) tube
  • Pref Vol:
    1.0 mL
  • Min Vol:
    0.2 mL
  • Fasting:
    No
  • Spec Collect:
    Do not use gel-barrier tubes for specimen collection
    Routine venipuncture
  • Spec Process:
    Clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum to separate plastic tube
  • Spec Store Transport:
    Refrigerated (48 hours) or frozen
  • Spec Stability:
    48 hours refrigerated (2-8°C)
    Greater than 48 hours, freeze (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles
  • Spec Reject:
    Serum not frozen within 48 hours of collection
    Sample collected in anticoagulant tube
  • Methodology:
    Enzyme-Linked Immunosorbent Assay (ELISA)
  • Use:
    Used to detect heparin-associated antibodies, which are found in some patients undergoing heparin therapy
  • Clinical Significance:
    Heparin is one of the most common causes of drug-induced thrombocytopenia. Heparin-induced thrombocytopenia (HIT) is subdivided into two groups. Type I is attributed to a direct interaction between heparin and platelets. Type I HIT is generally considered a benign condition and is not antibody-mediated. Type II HIT is of immunological origin and characterized by immune-mediated activation and clearance of platelets. It occurs 5-14 days after heparin exposure in 1-3% of patients and is associated with thrombosis. In untreated HIT, progression to thrombosis occurs in up to 50% of patients. Venous thromboembolism accounts for the majority of the thrombotic events but arterial thrombosis may also occur.
  • Reference Range:
    Anti-HEPARIN-PF4: Less than 0.40 O.D.

    Interpretive Data
    OD of the reaction greater or equal to 0.4 indicates the presence of a heparin-associated antibody.

  • Day Run:
    Mon-Sat
  • Time Run:
    11:00 am
  • Time Reported:
    5:00 pm
  • Test Type:
    COAGULATION

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