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Test Name:
Cardiolipin IgA Antibody


  • SBMF No:
    28104
  • Performance Lab Name:
    Immunology
  • Test Mnemonic:
    CARDA
  • CPT Code:
    86147
  • Also Known As:
    ACL IgA Antibody; Anticardiolipin IgA; Phospholipid IgA Antibody
  • Also See:
    28102 Cardiolipin IgG Antibody
    28103 Cardiolipin IgM Antibody
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Pref Vol:
    0.5 mL
  • Min Vol:
    0.3 mL
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum to separate plastic tube
    Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube
  • Spec Store Transport:
    Refrigerated (48 hours) or frozen
  • Spec Stability:
    8 hours room temperature (20-30°C)
    48 hours refrigerated (2-8°C)
    1 month frozen (-20°C) (avoid repeated freeze/thaw cycles)
  • Spec Reject:
    Gross hemolysis or lipemia
    Not frozen if more than 48 hours after collection
  • Spec Remarks:
    If specimen will not be received within 48 hours of collection, freeze serum and transport frozen
  • Methodology:
    Enzyme-Linked Immunosorbent Assay (ELISA)
  • Clinical Significance:

    Autoantibodies directed against certain phospholipids, including anti-cardiolipin (ACL) antibodies, have been shown to be associated with recurrent vascular thrombosis, recurrent spontaneous abortions and thrombocytopenia. This combination has been termed "Antiphospholipid Antibody Syndrome."

    ACL antibodies are similar to other antiphospholipid antibodies, Lupus Anticoagulants (LA), so-called because they can be seen in as many as 30% of patients with Systemic Lupus Erythematosus. ACL antibodies are also seen in patients with other autoimmune or connective tissue diseases, those undergoing chlorpromazine treatment, as well as patients with no known underlying diseases. Patients may demonstrate autoantibodies to either cardiolipin or lupus anticoagulants or both. Anticoagulant activity can be evaluated by the "Circulating Anticoagulant Detection Test."

    While most reports of cardiolipin antibodies center on IgG and/or IgM antibodies, some recent studies indicate that elevated levels of IgA Cardiolipin Antibodies are also frequently found in patients with SLE and related disorders. In these studies IgA values were higher in patients with vascular complications and thrombocytopenia.

  • Reference Range:
      Cardiolipin IgA Antibody
        Less than 12 APL – Negative
        12-20 APL – Intermediate
        Greater than 20 APL – Positive

        APL = IgA Phospholipid Units
      Interpretive Data:
        A "positive" result indicates the presence of cardiolipin IgA antibodies and can be used in conjunction with other serological tests and clinical findings to aid in assessing the risk of thrombosis in individuals with Systemic Lupus Erythematosus (SLE) or lupus-like disorders
  • Additional Test Info:

    Online LIS Test Build Information is not available for this test. For a complete test mix, contact interface support at ITDMUS@sbmf.org.

  • Day Run:
    Tue, Fri
  • Time Run:
    8:00 am
  • Time Reported:
    5:00 pm, next day
  • Test Type:
    IMMUNOLOGY