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Test Name:
Parasitology Stain, by Modified Acid-Fast, Feces
- SBMF No:
27018 - Performance Lab Name:
Microbiology - Test Mnemonic:
PARA STAIN - ABN:
Not required - CPT Code:
87015; 87206 - LOINC Code:
10853-0 - Also Known As:
Stain for Cryptosporidium, Cyclospora, and/or Isospora - Also See:
27010 – Ova & Parasites, Routine, Feces (Cryptosporidium and Giardia Antigens)
45497 – Entamoeba histolytica Antigen, by EIA, Feces
27012 – Ova & Parasites, Travel History/Immunocompromised/Specified Parasite(s) - Spec Type:
Feces, fresh, random - Spec Container:
Yellow cap SAF collection container (contains sodium acetate, acetic acid and formalin)
Also acceptable, but not preferred: Pink capped ParaPak collection container (contains 10% buffered neutral formalin) - Pref Vol:
Sufficient fecal sample to cause solution in container to rise even with "fill line" - Patient Prep:
If necessary, acceptable to obtain samples with warm saline enema solutions or Fleet Phospho-Soda
Do not obtain samples by using enemas containing mineral oil, bismuth, iron, or magnesium compounds
Wait at least 1 week after barium procedures or laxatives before collecting O&P samples - Spec Collect:
Add amount of feces to container sufficient to cause solution level to rise even with container "fill line"
Do not over-fill – solution level must be at indicated "fill line"
Mix feces and solution in container by gentle inversion
Refer to: P-1003 Feces (Stool) Collection and Transport - Spec Store Transport:
Room temperature - Spec Stability:
2 weeks room temperature (20-30°C) - Spec Reject:
Sample not preserved in O&P collection container
Vial over-filled with sample (solution above "fill line")
Multiple samples collected less than 24 hours apart
SBMF does not accept animal samples for testing - Spec Remarks:
Do not collect more than 1 sample within 24 hours
– Multiple sample orders (3 samples recommended): wait 24-48 hours between collection of each sample
– Multiple samples collected less than 24 hours apart will be pooled and processed as a single specimen
O&P examination is not recommended for inpatients after fourth day of hospitalization - Methodology:
Microscopic Examination - Use:
Diagnose cryptosporidiosis, cyclosporiasis and/or isosporiasis
For patients with suspected Cryptosporidium sp. infection, stool antigen testing is the optimal test method for diagnosis - Clinical Significance:
Cryptosporidium sp. is recognized as a cause of severe diarrhea in immunodeficient. In addition, it can cause transient diarrhea in immunocompetent individuals. - Reference Range:
No Cryptosporidium oocysts observed - Additional Test Info:
One negative result does not rule out a parasitic infection
Collect 1-2 more samples with 24-48 hours betwen each collection - Day Run:
Varies - Time Run:
Day shift - Time Reported:
Daily - Test Type:
PARASITE
