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Test Name:
CBC with Automated Differential

• SBMF No:
  25517
• Performance Lab Name:
  Hematology
• Test Mnemonic:
  ZCBCH
• ABN:
  May be required – Medical Necessity
• CPT Code:
  85025
• LOINC Code:
  770-8; 6690-2; 789-8; 718-7; 4544-3; 787-2; 785-6; 786-4; 788-0; 777-3; 736-9; 5905-5; 713-8; 706-2; 751-8; 731-0; 742-7; 711-2; 704-7
• Test Includes:
  White Blood Cells (WBC)
  Red Blood Cells (RBC)
  Hemoglobin (HGB)
  Hematocrit (HCT)
  Mean Corpuscular Volume (MCV)
  Mean Corpuscular Hemoglobin (MCH)
  Mean Corpuscular Hemoglobin Concentration (MCHC)
  RBC Distribution Width (RDW)
  Platelets (PLT)
  Differential, Automated
• Also Known As:
  Complete Blood Count with Differential
• Also See:
  25014 CBC without Differential
• Spec Type:
  Whole blood
• Spec Container:
  Lavender top (EDTA) tube
• Pref Vol:
  5.0 mL
• Min Vol:
  1.0 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
  Tube must fill to one-half, or greater, of vacuum capacity
  Immediately after collection, gently invert tube to mix
• Spec Process:
  Do not centrifuge
  Do not remove plasma from cells
• Spec Store Transport:
  Refrigerated
  Optimally, specimen should be received for testing within 24 hours of collection time
• Spec Stability:
  8 hours room temperature (20-30°C)
  24 hours refrigerated (2-8°C)
  Frozen (-20°C): Unacceptable
• Spec Reject:
  Collection tube not filled at least ½ of vacuum capacity
  Clotted, hemolyzed, or centrifuged sample
• Spec Remarks:
  NOTE: A manual differential may be reflexed to complete the order as needed
• Methodology:
  Pulse Measurement technology, Hydrodynamic Focusing, RF (Radio-Frequency) detection, and DC (Direct Current) detection.
• Use:
  Evaluate anemia, leukemia, reaction to inflammation and infections, peripheral blood cellular characteristics, state of hydration and dehydration, polycythemia, hemolytic disease of the newborn, inherited disorders of red cells, white cells, and platelets; manage chemotherapy decisions; determine qualitative and quantitative variations in white cell numbers and morphology; morphology of red cells and platelet evaluation
• Clinical Significance:
  

  To rule out anemia, leukemia, inflammation, and infection
  To view peripheral blood cellular characteristics and quantitate red blood cells (RBC), white blood cells (WBC), and platelets.

  Also see:
  

• Reference Range:
  WBC: 4.0-11.0 K/μL
  RBC, Male: 3.9-5.9 M/μL
  RBC, Female: 3.7-5.4 M/μL
  Hgb, Male: 13.0-17.3 g/dL
  Hgb, Female: 11.4-15.4 g/dL
  Hct, Male: 39-53%
  Hct, Female: 35-47%
  MCV: 81-100 fl
  MCH, Male: 27-34 pg
  MCH, Female: 25-34 pg
  MCHC: 30-36 g/dL
  Platelets: 130-470 K/μL
  RDW: 12.2-15.2%
  Differential:
    Neutrophils: 37-77% (absolute result 1.67-8.47 x 10³/mL)
    Lymphocytes: 23-44% (absolute result 1.03-4.84x 10³/mL)
    Monocytes: 4-13% (absolute result 0.27-0.98 x 10³/mL)
    Eosinophils: 1-6% (absolute result 0.11-0.55 x 10³/mL)
    Basophils: 0-1% (absolute result 0.02-1.10 x 10³/mL)
• Critical Low Value:
  WBC: less than or equal to (≤) 2.5 K/μL
  Hgb: less than or equal to (≤) 8.0 g/dL
  Platelets: less than or equal to (≤) 30.0 K/μL
  Hct: less than or equal to (≤) 24.0%L
• Critical High Value:
  WBC: equal to or greater than (≥) 30.0 K/μL
  Hgb: equal to or greater than (≥) 20.0 g/dL
  Platelets: equal to or greater than (≥) 1,000 K/μL
  Hct: equal to or greater than (≥) 60.0%
• Additional Test Info:
  

  If specimen is greater than 24 hours old, automated differential results may be affected; however, differential will be performed, indicating specimen age and a footnote will be attached to the report that reads: "Diff may be inaccurate due to specimen integrity"

  Pathologist will review all results (if no prior diagnosis is consistent with patient's results) when:
       – WBC results are: less than 1.5 K/μL or greater than 30 K/μL
       – RBC results are: less than 2.5 M/μL or greater than 6.5 M/μL
       – Hgb results are: less than 4.0 g/dL or greater than 20.0 g/dL
       – MCV results are: less than 60.0 fl or greater than 120.0 fl
       – Platelet results are: less than 10 K/μL or greater than 1,000 K/μL

• Day Run:
  Sun-Sat (daily)
• Time Run:
  As received
• Time Reported:
  4 hours
• Test Type:
  HEMATOLOGY