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Test Name:
Hemoglobin and Hematocrit, Blood
- SBMF No:
25316 - Performance Lab Name:
Automated Lab - Test Mnemonic:
H/H - ABN:
May be required – Medical Necessity - CPT Code:
85014; 85018 - LOINC Code:
718-7; 4544-3 - Test Includes:
Hemoglobin
Hematocrit - Also Known As:
Hematocrit and Hemoglobin
H&H - Also See:
25015 CBC with Differential
25014 CBC without Differential - Spec Type:
Whole blood - Spec Container:
Lavender top (EDTA) tube - Pref Vol:
5.0 mL - Min Vol:
1.0 mL - Fasting:
No - Spec Collect:
Routine venipuncture
Tube must fill to one-half, or greater, of vacuum capacity
Immediately after collection, gently invert tube to mix - Spec Process:
Do not centrifuge
Do not remove plasma from cells - Spec Store Transport:
Room temperature or refrigerated
Note: Optimally, specimen should be received for testing within 24 hours of collection time - Spec Stability:
24 hours room temperature (20-30°C)
24 hours refrigerated (2-8°C) - Spec Reject:
Collection tube not filled at least ½ of vacuum capacity
Sample contains clots
Hemolyzed or clotted sample
Centrifuged sample - Methodology:
Pulse Measurement and Hydrodynamic Focusing - Use:
Evaluate anemia, blood loss, hemolytic anemia, polycythemia and other conditions - Clinical Significance:
Hemoglobin is used to investigate suspected anemia or erythrocytosis.
Anemia is defined as a hemoglobin value below normal for age and gender.
Further investigation of the cause of anemia is guided by clinical features, blood film and red cell indices (MCV, MCH, MCHC).
The hemoglobin is elevated in erythrocytosis.
Hematocrit is used in the assessment of anemia and erythrocytosis or to monitor hemodilution or hemoconcentration.
The hematocrit is reduced in anemia; increased in erythrocytosis.
In patients with erythrocytosis, the hematocrit correlates with blood viscosity. - Reference Range:
Hemoglobin:
– Male, Adult: 13.0-17.3 g/dL
– Female, Adult: 11.4-15.4 g/dL
Hematocrit:
– Male, Adult: 39-53%
– Female, Adult: 35-47%
– Newborns (M/F): 44% to 64%
– 3 months (M/F): 35% to 49%
– 2 years (M/F): 30% to 40%
– 6 years (M/F): 31% to 43%
Note: In general, centrifuged hematocrits are 2% to 3% higher than automated hematocrits, due to plasma trapping - Critical Low Value:
Adult:
– Hemoglobin: 8.0 g/dL
– Hematocrit: 24.0 %;
Newborn:
– Hemoglobin: 10.0 g/dL
– Hematocrit: 24% - Critical High Value:
Adult:
– Hemoglobin: 20.0 g/dL
– Hematocrit: 60.0% - Additional Test Info:
A pathologist will review results when hemoglobin is less than 4.0 g/dL and greater than 20.0 g/dL - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
HEMATOLOGY
