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Test Name:
Fibrinogen
- SBMF No:
25223 - Performance Lab Name:
Hem-Coag - Test Mnemonic:
FIB - ABN:
Not required - CPT Code:
85384 - LOINC Code:
3255-7 - Spec Type:
Platelet-poor plasma (PPP) - Spec Container:
Light blue top (3.2% buffered sodium citrate) tube - Pref Vol:
1.0 mL (Two aliquots, 0.5 mL each) - Fasting:
No - Patient Prep:
Collect prior to initiation of anticoagulant therapy - Spec Collect:
- Routine venipuncture
- • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
• Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
• Fill light blue top tube to stated tube volume
• If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
• If multiple coagulation studies are requested, submit additional samples for each coagulation test
• Immediately after collection, gently invert tube to mix
- Spec Process:
- Separate plasma from cells immediately
- • Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• NOTE: Buffy coat layer of sample must be avoided
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
• Immediately freeze PPP samples
- Spec Store Transport:
Frozen - Spec Stability:
4 hours room temperature (20-30°C)
2 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Citrate tube underfilled
Sample clotted or hemolyzed
Plasma not frozen within 4 hours of collection - Spec Remarks:
Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results - Methodology:
Photo-Optical Clot Detection - Use:
Identify congenital afibrinogenemia, disseminated intravascular coagulation, and fibrinolytic activity - Clinical Significance:
Dysfibrinogenemias may be associated with bleeding or thrombosis. This may be suspected in patients who have prolonged thrombin or reptilase clotting times, and can be confirmed by measuring simultaneous functional and antigenic fibrinogen values. An antigenic value 65% greater than the functional value is diagnostic of dysfibrinogenemia. Dysfibrinogens may be inherited or acquired in association with liver disease. - Reference Range:
180-350 mg/dL - Critical Low Value:
50 mg/dL - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
COAGULATION
