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Test Name:
T-Cells, CD4/CD8; B-Cells, by Flow Cytometry


  • SBMF No:
    25218
  • Performance Lab Name:
    Flow Cytometry/Molecular Pathology
  • Test Mnemonic:
    IMM DEFPED
  • ABN:
    Not required
  • CPT Code:
    86355; 86359; 86360
  • Test Includes:
    T-Cell Markers:
         – CD3 (T-Cells)
         – CD4 (Helper Cells)
         – CD8 (Suppressor Cells)
    CD19 (B-Cell Marker)
    Helper to Suppressor Cell Ratio (calculated)
  • Also Known As:
    Lymphocyte Panel, Partial, Pediatric
    PED PANEL
    Lymphocyte Subset Panel
    Lymphocyte Subpopulation Analysis
  • Also See:
    25217 – T-Cells, CD4/CD8, by Flow Cytometry
    25215 – T-Cells, CD4/CD8; B-Cells; NK-Cells, by Flow Cytometry
  • Spec Type:
    Whole blood
  • Spec Container:
    2 tubes: Pale yellow top (ACD) tube -plus- lavender top (EDTA) tube
  • Pref Vol:
    7.0 mL ACD whole blood - plus - 5.0 mL EDTA whole blood
  • Min Vol:
    Contact SBMF Flow Cytometry department for specific instructions
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Do not centrifuge
    Do not remove plasma from cells
    Do not transfer blood to other containers
    Specimen cannot be frozen
  • Spec Store Transport:
    Room temperature
    Optimally, transport to SBMF within 12 hours of collection
  • Spec Stability:
    EDTA samples: 30 hours room temperature (20-30°C)
    ACD samples: 3 days room temperature (20-30°C)
  • Spec Reject:
    Not collected in appropriate container
    Hemolyzed sample or contains clots
    Sample refrigerated
    Sample received more than 72 hours after collection
  • Methodology:
    Flow Cytometry
  • Use:
    Used to characterize some forms of immunodeficiency and autoimmune diseases
  • Clinical Significance:
    Total T- and B-lymphocyte percentages may be used to characterize some forms of immunodeficiency and autoimmune diseases. When monoclonal antibody reagents are added to human whole blood, the flourochrome-labeled antibodies bind specifically to antigens on the surface of leukocytes. Using the CD45 antigen and side scatter, cells can be grouped and the cells of interest isolated for analysis. This method is superior to two-color methodology in that the lymphocyte gate and recovery are both assumed to be 100%.
  • Reference Range:
    Total White Cell Count: 4,500-11,000/μL
    Total Lymphocyte Count: 800-4,800/μL
    Absolute B-Cells (CD19+): 100-430/μL
    % B-Cells (CD19+): 6-23%
    Absolute (CD3+): 720-2,330/μL
    % Lymphocytes (CD3+): 59-85%
    Absolute (CD3+CD4+): 430-1,760
    % Lymphocytes (CD3+CD4+): 29-61%
    Absolute (CD3+CD8+): 170-1,050/μL
    % Lymphocytes (CD3+CD8+): 11-38%
    Helper/Suppressor Ratio: greater than 0.9
  • Day Run:
    Tue, Thu, Sat
  • Time Run:
    5:00 pm
  • Time Reported:
    24 hours
  • Test Type:
    FLOW

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